+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Distinct immunomodulation of bone marrow-derived dendritic cell responses to Lactobacillus plantarum WCFS1 by two different polysaccharides isolated from Lactobacillus rhamnosus LOCK 0900



Distinct immunomodulation of bone marrow-derived dendritic cell responses to Lactobacillus plantarum WCFS1 by two different polysaccharides isolated from Lactobacillus rhamnosus LOCK 0900



Applied and Environmental Microbiology 80(20): 6506-6516



The structures of polysaccharides (PS) isolated from Lactobacillus rhamnosus LOCK 0900 and results from stimulation of mouse bone marrow-derived dendritic cells (BM-DC) and human embryonal kidney (HEK293) cells stably transfected with Toll-like receptors (TLR) upon exposure to these antigens were studied. L. rhamnosus LOCK 0900 produces PS that differ greatly in their structure. The polymer L900/2, with a high average molecular mass of 830 kDa, is a branched heteropolysaccharide with a unique repeating unit consisting of seven sugar residues and pyruvic acid, whereas L900/3 has a low average molecular mass of 18 kDa and contains a pentasaccharide repeating unit and phosphorus. Furthermore, we found that both described PS neither induce cytokine production and maturation of mouse BM-DC nor induce signaling through TLR2/TLR4 receptors. However, they differ profoundly in their abilities to modulate the BM-DC immune response to the well-characterized human isolate Lactobacillus plantarum WCFS1. Exposure to L900/2 enhanced interleukin-10 (IL-10) production induced by L. plantarum WCFS1, while in contrast, L900/3 enhanced the production of IL-12p70. We conclude that PS, probably due to their chemical features, are able to modulate the immune responses to third-party antigens. The ability to induce regulatory IL-10 by L900/2 opens up the possibility to use this PS in therapy of inflammatory conditions, such as inflammatory bowel disease, whereas L900/3 might be useful in reverting the antigen-dependent Th2-skewed immune responses in allergies.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052663321

Download citation: RISBibTeXText

PMID: 25107979

DOI: 10.1128/aem.02104-14


Related references

Polysaccharides L900/2 and L900/3 isolated from Lactobacillus rhamnosus LOCK 0900 modulate allergic sensitization to ovalbumin in a mouse model. Microbial Biotechnology 10(3): 586-593, 2017

Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime. Food and Function 7(6): 2840-2848, 2016

Lactobacillus rhamnosus LA68 and Lactobacillus plantarum WCFS1 differently influence metabolic and immunological parameters in high fat diet-induced hypercholesterolemia and hepatic steatosis. Food and Function 6(2): 558-565, 2015

Mg 2+ improves the thermotolerance of probiotic Lactobacillus rhamnosus GG, Lactobacillus casei Zhang and Lactobacillus plantarum P-8. Letters in Applied Microbiology 64(4): 283-288, 2017

The innovative potential of Lactobacillus rhamnosus LR06, Lactobacillus pentosus LPS01, Lactobacillus plantarum LP01, and Lactobacillus delbrueckii Subsp. delbrueckii LDD01 to restore the "gastric barrier effect" in patients chronically treated with PPI: a pilot study. Journal of Clinical Gastroenterology 46 Suppl (): S18-S26, 2013

Eruca sativa might influence the growth, survival under simulated gastrointestinal conditions and some biological features of Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus strains. International Journal of Molecular Sciences 15(10): 17790-17805, 2014

Production of succinate by Lactobacillus plantarum, Lactobacillus casei and Lactobacillus rhamnosus. Abstracts of the General Meeting of the American Society for Microbiology 99: 509, 1999

Physiological responses to folate overproduction in Lactobacillus plantarum WCFS1. Microbial Cell Factories 9: 100, 2010

Heat-inactivated Lactobacillus rhamnosus and Lactobacillus delbrueckii induce efficient maturation and differential cytokine production in human monocyte derived dendritic cells. Food and Agricultural Immunology 24(1): 95-109, 2013

Distinct effects of lactobacillus rhamnosus and klebsiella pneumoniae on final dendritic cell function. Digestive Disease Week Abstracts & Itinerary Planner : Abstract No S1112, 2003

Lactobacillus plantarum ZS2058 and Lactobacillus rhamnosus GG Use Different Mechanisms to Prevent Salmonella Infection in vivo. Frontiers in Microbiology 10: 299, 2019

Survival of freeze-dried Lactobacillus plantarum and Lactobacillus rhamnosus during storage in the presence of protectants. Biotechnology letters 24(19): 1587-1591, 2002

Probiotic strains Lactobacillus plantarum 299V and Lactobacillus rhamnosus GG as starter cultures for fermented sausages. LWT - Food Science and Technology 54(1): 51-56, 2013

Serological identification of lactic acid bacteria lactobacillus casei lactobacillus acidophilus lactobacillus helveticus lactobacillus plantarum lactobacillus bulgaricus. Mikrobiologiya 36(4): 675-683, 1967

Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera. International Journal of Systematic and Evolutionary Microbiology 64(Pt 9): 3109-3119, 2014