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EGFR Mutations Detection in Non-small Cell Lung Cancer Tissues by Real-time PCR and DNA Sequencing.



EGFR Mutations Detection in Non-small Cell Lung Cancer Tissues by Real-time PCR and DNA Sequencing.



Zhongguo Fei Ai Za Zhi 12(12): 1255-1259



Small molecule tyrosine kinase inhibitors (TKIs), such as gefitinib and erlotinib that target the kinase domain of epidermal growth factor receptor (EGFR) are making successful progression for advanced non-small cell lung cancer patients treatment. The growing evidences revealed that EGFR exon 19 and 21 mutation status in NSCLC patients was correlated with the outcome for EGFR-TKI treatment. In this study, two methods of Real-time PCR and DNA sequencing were compared to detected EGFR exon 19 and 21 mutations. EGFR exon19 mutation del-E746-A750 and exon 21mutation L858R were detected by Real-time PCR and DNA sequencing in 103 NSCLC patients. Chi-square test was used to analyze the consistance. There was no significant difference between the two methods. However, Real-time PCR was more convenient and sensitive compared to DNA sequencing. Real-time PCR was more suitable for clinical testing than DNA sequencing.

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Accession: 052747918

Download citation: RISBibTeXText

PMID: 20723379

DOI: 10.3779/j.issn.1009-3419.2009.12.06


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