+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Effect of dietary nonstructural carbohydrate content on activation of 5'-adenosine monophosphate-activated protein kinase in liver, skeletal muscle, and digital laminae of lean and obese ponies



Effect of dietary nonstructural carbohydrate content on activation of 5'-adenosine monophosphate-activated protein kinase in liver, skeletal muscle, and digital laminae of lean and obese ponies



Journal of Veterinary Internal Medicine 28(4): 1280-1288



In EMS-associated laminitis, laminar failure may occur in response to energy failure related to insulin resistance (IR) or to the effect of hyperinsulinemia on laminar tissue. 5'-Adenosine-monophosphate-activated protein kinase (AMPK) is a marker of tissue energy deprivation, which may occur in IR. To characterize tissue AMPK regulation in ponies subjected to a dietary carbohydrate (CHO) challenge. Twenty-two mixed-breed ponies. Immunohistochemistry and immunoblotting for total AMPK and phospho(P)-AMPK and RT-qPCR for AMPK-responsive genes were performed on laminar, liver, and skeletal muscle samples collected after a 7-day feeding protocol in which ponies stratified on body condition score (BCS; obese or lean) were fed either a low-CHO diet (ESC + starch, approximately 7% DM; n = 5 obese, 5 lean) or a high-CHO diet (ESC + starch, approximately 42% DM; n = 6 obese, 6 lean). 5'-Adenosine-monophosphate-activated protein kinase was immunolocalized to laminar keratinocytes, dermal constituents, and hepatocytes. A high-CHO diet resulted in significantly decreased laminar [P-AMPK] in lean ponies (P = .03), but no changes in skeletal muscle (lean, P = .33; obese, P = .43) or liver (lean, P = .84; obese, P = .13) [P-AMPK]. An inverse correlation existed between [blood glucose] and laminar [P-AMPK] in obese ponies on a high-CHO diet. Laminar tissue exhibited a normal response to a high-CHO diet (decreased [P-AMPK]), whereas this response was not observed in liver and skeletal muscle in both lean (skeletal muscle, P = .33; liver, P = .84) and obese (skeletal muscle, P = .43; liver, P = .13) ponies.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052802788

Download citation: RISBibTeXText

PMID: 24750267

DOI: 10.1111/jvim.12356


Related references

Genistein promotes insulin action through adenosine monophosphate–activated protein kinase activation and p7 ribosomal protein S6 kinase 1 inhibition in the skeletal muscle of mice fed a high energy diet. 2012

Genistein promotes insulin action through adenosine monophosphate-activated protein kinase activation and p70 ribosomal protein S6 kinase 1 inhibition in the skeletal muscle of mice fed a high energy diet. Nutrition Research 32(8): 617-625, 2013

Metformin promotes induction of lipoprotein lipase in skeletal muscle through activation of adenosine monophosphate-activated protein kinase. Metabolism: Clinical and Experimental 58(10): 1408-1414, 2009

Atorvastatin and pitavastatin enhance lipoprotein lipase production in L6 skeletal muscle cells through activation of adenosine monophosphate-activated protein kinase. Metabolism: Clinical and Experimental 61(10): 1452-1460, 2012

Rapid activation by 3,5,3'-L-triiodothyronine of adenosine 5'-monophosphate-activated protein kinase/acetyl-coenzyme a carboxylase and akt/protein kinase B signaling pathways: relation to changes in fuel metabolism and myosin heavy-chain protein content in rat gastrocnemius muscle in vivo. Endocrinology 149(12): 6462-6470, 2008

Evidence for new alleles in the protein kinase adenosine monophosphate-activated c3-subunit gene associated with low glycogen content in pig skeletal muscle and improved meat quality. Genetics 159(3): 51-62, 2001

Post-meal responses of elongation factor 2 (eEF2) and adenosine monophosphate-activated protein kinase (AMPK) to leucine and carbohydrate supplements for regulating protein synthesis duration and energy homeostasis in rat skeletal muscle. Nutrients 4(11): 1723-1739, 2013

Evidence for new alleles in the protein kinase adenosine monophosphate-activated gamma(3)-subunit gene associated with low glycogen content in pig skeletal muscle and improved meat quality. Genetics 159(3): 1151-1162, 2001

Adenosine monophosphate-activated protein kinase involved in variations of muscle glycogen and breast meat quality between lean and fat chickens. Journal of Animal Science 86(11): 2888-2896, 2008

Adenosine 5'-monophosphate-activated protein kinase regulation of fatty acid oxidation in skeletal muscle. Endocrinology 149(3): 935-941, 2008

Berberine-induced activation of 5'-adenosine monophosphate-activated protein kinase and glucose transport in rat skeletal muscles. Metabolism: Clinical and Experimental 59(11): 1619-1627, 2010

Enhanced Production of Adenosine Triphosphate by Pharmacological Activation of Adenosine Monophosphate-Activated Protein Kinase Ameliorates Acetaminophen-Induced Liver Injury. Molecules and Cells 38(10): 843-850, 2016

Isolation of a Unique Phosphopeptide from Skeletal-Muscle Phosphorylase Kinase, Labelled During Activation by Adenosine 3:5-Cyclic Monophosphate-Dependent Protein Kinase. Biochemical Society Transactions 2(1): 82-83, 1974

Activation of rabbit skeletal muscle adenosine-3':5'-monophosphate-dependent protein kinase by agitation. Biochemical and Biophysical Research Communications 76(4): 1116-1123, 1977

Angiotensin II inhibits glucose uptake of skeletal muscle via the adenosine monophosphate-activated protein kinase pathway. Journal of the American Society of Hypertension 1(4): 251-255, 2007