+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Effects of diagnostic ultrasound on HSP70 expression in chorionic villi in rats during early pregnancy and the role of HSP70 in apoptosis in chorionic villi



Effects of diagnostic ultrasound on HSP70 expression in chorionic villi in rats during early pregnancy and the role of HSP70 in apoptosis in chorionic villi



International Journal of Molecular Medicine 32(5): 1085-1092



The aim of this study was to investigate the effects of diagnostic ultrasound on chorionic villi during early pregnancy in rats through the examination of heat shock protein 70 (HSP70) expression and apoptosis in chorionic villi. Using TUNEL, RT-PCR, western blot analysis and immunohistochemistry, the extent of apoptosis and the gene/protein expression of HSP70, Bcl-2 and Bax was determined in the samples of the chorionic villus from the rats during early pregnancy. Compared with the unexposed group, there were clear signs that apoptosis had occurred in the villi exposed to ultrasound for 30 min. The expression of HSP70 and Bax in the villi gradually increased with the extended duration of exposure to ultrasound, whereas the expression of Bcl-2 gradually decreased. Bcl-2 expression did not differ significantly between the group exposed for 10 min and the group exposed for 20 min. Taken together, our data demonstrate that HSP70 may protect chorionic villi exposed to ultrasound by inhibiting apoptosis; however, its protective effects are limited over the extended duration of exposure to ultrasound. Thus, the prudent use of ultrasound during the early stages of pregnancy should be advocated.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052862286

Download citation: RISBibTeXText

PMID: 24026294

DOI: 10.3892/ijmm.2013.1489


Related references

Preliminary study of the effects of diagnostic ultrasound in early pregnant women on the chorionic villi. Hua Xi Yi Ke Da Xue Xue Bao 18(1): 71-74, 1987

Preliminary study of effects of diagnostic ultrasound in early pregnant women on chorionic villi. Journal of West China University of Medical Sciences 18(1): 71-74, 1987

Interpreting mosaicism in chorionic villi: results of a monocentric series of 1001 mosaics in chorionic villi with follow-up amniocentesis. Prenatal Diagnosis 35(11): 1117-1127, 2015

Dynamic of apoptosis changes in early normal pregnancy chorionic villi and decidua. Placenta 20(5-6): A 14, 1999

Vaginosonographic guided chorionic villi needle biopsy (transvaginal chorionic villi sampling). European Journal of Obstetrics Gynecology and Reproductive Biology 23(5-6): 315-319, 1986

Effects of mifepristone on proliferation and apoptosis in early chorionic villi. Zhonghua Fu Chan Ke Za Zhi 33(5): 268-270, 1998

The villi test (chorionic villi biopsy) in the 2d and 3d trimester of pregnancy. Nederlands Tijdschrift Voor Geneeskunde 132(17): 765-767, 1988

Relationship of SOCS3 and TGF-β with IDO expression in early pregnancy chorionic villi and decidua. Experimental and Therapeutic Medicine 14(5): 4817-4824, 2017

Apoptosis and expression of relative genes in early pregnant chorionic villi and decidua. Zhonghua Fu Chan Ke Za Zhi 37(4): 220-222, 2002

Expression of angiogenesis- and apoptosis-related genes in chorionic villi derived from recurrent pregnancy loss patients. Molecular Reproduction and Development 66(1): 24-31, 2003

Expression of telomerase activity, MIB-1 and Bcl-2 in human chorionic villi from early and term normal pregnancy. Placenta 21(7): A 33, 2000

Expression of miRNA-4497 in human chorionic villi from early recurrent miscarriage and the influence on apoptosis. Zhonghua Yi Xue Za Zhi 95(46): 3737-3740, 2015

Ultrastructural changes of human chorionic villi after exposure to diagnostic ultrasound. Xi'an Yike Daxue Xuebao 11(1): 79-81, 1990

Cytogenetic analysis of chorionic villi for prenatal diagnosis: an ACC collaborative study of U.K. data. Association of Clinical Cytogeneticists Working Party on Chorionic Villi in Prenatal Diagnosis. Prenatal Diagnosis 14(5): 363-379, 1994

Ultrastructure of early human chorionic villi obtained by chorionic villous sampling. Singapore Journal of Obstetrics & Gynaecology 20(2): 66-75, 1989