+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Effects of human fecal flora on intestinal morphology and mucosal immunity in human flora-associated piglet



Effects of human fecal flora on intestinal morphology and mucosal immunity in human flora-associated piglet



Scandinavian Journal of Immunology 69(3): 223-233



Human flora-associated (HFA) piglet model was established to examine the effects of gut microbes from a different donor species on the intestinal morphology and mucosal immunity. Newborn germ-free piglets, obtained by caesarean section, were orally inoculated with a human and a porcine faecal suspension, and artificially fed to establish a HFA group (n = 7) and pig flora-associated (PFA) group (n = 7), respectively. All pigs were killed 6 weeks later. Tissue samples from duodenum, jejunum, ileum and colon were collected and studied by histochemistry and immunohistochemistry methods for intestinal morphological analyses and detection of immunocompetent cells. In summary, both groups of pigs performed well but HFA pigs had a somewhat better daily weight gain, and their jejunal villus height and crypt depth were significantly higher. In comparison with PFA pigs, the number of intraepithelial lymphocytes in jejunum was lower but the number of goblet cells containing neutral mucins was significantly increased in HFA pigs. No difference was observed in the number of mast cells. The areas of IgA producing cells and CD4(+) T cells in the jejunum and IgG producing cells in the small intestine were significantly higher in HFA pigs. However, the areas of MHC class II expressing cells were significantly increased in the duodenum and colon. Additionally, the amount of Bifidobacteria spp. was significantly higher in HFA pigs. This study confirms that the composition of gut microbes differentially affects the host intestinal mucosal immunity and suggests that commensal bacteria have great effects on intestinal health and development.

(PDF emailed within 0-6 h: $19.90)

Accession: 052870234

Download citation: RISBibTeXText

PMID: 19281534

DOI: 10.1111/j.1365-3083.2008.02211.x


Related references

The Metschnikoff theory the and 2730 influence of diet on the intestinal flora, growth, propagation, behavior and blood picture of the white rat II The nature of the intestinal contents, ie, the feces, and the formation of the intestinal or fecal flora with a preponderantly vegetable, carbohydrate-rich diet, and the conditions of change of the intestinal flora to a lactic acid bacterial flora. 1929

Effects of human intestinal flora on plasma and caecal isoflavones, and effects of isoflavones on the composition and metabolism of flora in human flora-associated (HFA) mice. Microbial Ecology in Health and Disease 16(1): 18-22, 2004

Effects of dietary supplements on the composition of fecal flora of human-flora-associated (HFA) mice. Bifidobacteria and Microflora 13(1): 1-7, 1994

Effects of Depolymerised Sodium Alginate on Human Fecal Flora and Intestinal Environment. Nippon Suisan Gakkaishi 60(1): 85-90, 1994

Studies on the intestinal flora of swine. 3. Effects of fermented-Chlorella diet on fecal flora. Nihon Juigaku Zasshi. Japanese Journal of Veterinary Science 30(5): 281-287, 1968

Comparison of the in vitro metabolism of isoflavones by fecal flora from human flora-associated mice and human. Journal of the science of food and agriculture15 86(10): 1567-1570, 2006

Effect of the ingestion of wheat bran on the fecal microbial flora of human donors and of recipient gnotoxenic mice, and on the barrier effects exerted by these flora against various potentially pathogenic microorganisms. Annales de Microbiologie 135a(2): 303-318, 1984

Degradation of human intestinal glycosphingolipids by extracellular glycosidases from mucin-degrading bacteria of the human fecal flora. Journal of Biological Chemistry 263(22): 10790-8, 1988

Selective antimicrobial modulation of the intestinal tract by norfloxacin in human volunteers and in gnotobiotic mice associated with a human fecal flora. Antimicrobial Agents and ChemoTherapy 29(6): 1047-1052, 1986

Human flora-associated (HFA) animals as a model for studying the role of intestinal flora in human health and disease. Current Issues in Intestinal Microbiology 6(2): 69-75, 2005

Significance of fecal medium for human intestinal micro flora. Sasaki, S , A Ozawa And K Hashimoto (Ed ) Recent Advances in Germfree Research; Proceedings Of The 7th International Symposium on Gnotobiology, June 29-July 3, 1981, Tokyo, Japan Xviii+776p Tokai Univeristy Press: Shinjuku, Tokyo, Japan Illus Maps : p293-302, 1982

Human fecal flora: the normal flora of 20 Japanese-Hawaiians. Applied Microbiology 27(5): 961-979, 1974

IV Immunity and tolerance to normal intestinal microbial flora The influence of normal microbial flora on the development of chronic mucosal inflammation. Research in Immunology 148(8-9): 567-576, Oct -Dec , 1997 ( ), 1998

Studies of intestinal micro flora iii the microbial flora of human small intestinal mucosa and fluids bacteria. Gastroenterology 53(6): 868-873, 1967

Analysis of intestinal flora responsible for the development of the intestinal immune system and establishment of both human flora and the segmented filamentous bacteria-associated mouse. Journal Of Intestinal Microbiology: 2, 109-114, 2008