+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Effects of inhibited IGF-IR expression on proliferation and apoptosis of human hepatocellular carcinoma cell lines

Effects of inhibited IGF-IR expression on proliferation and apoptosis of human hepatocellular carcinoma cell lines

Zhonghua Gan Zang Bing Za Zhi 21(5): 376-380

ISSN/ISBN: 1007-3418

PMID: 24025141

To investigate the therapeutic value of inhibiting the expression of insulin-like growth factor-I receptor (IGF-IR) using picropodophyllin (PPP) by studying the effects on proliferative and metastatic potentials of human hepatocellular carcinoma (HCC) using an in vitro cultured cell system. IGF-IR expression in human HCC cell lines (Bel-7404, Bel-7402, HepG2, and Huh-7) and human hepatocytes (L02) was assessed at baseline (pre-treatment) and after PPP treatment by western blotting. Changes in cell cycle were analyzed by flow cytometry and in cell viability by sulforhodamine B staining. Early apoptosis was detected by annexin-V/FITC and propidium iodide double-staining assay. Caspase-3/7 activity was suppressed by z-VAD-FMK and analyzed by homogeneous luminescence assay. Effects on cell motility were tested by wound-scratch test. Between-group differences were assessed by t-test or one-way analysis of variance. IGF-IR was markedly up-regulated in all HCC cell lines (vs. non-hepatoma hepatocytes). HCC cells with PPP-inhibited IGF-IR showed time-dependent decreases in cell motility and viability. After treatment with PPP for 24 hours, the proportion of HCC cells in G1 phase was 2.1% +/- 0.4%, in S phase was 11.0% +/- 0.7%, and in G2/M phase was 87.1% +/- 0.6%, and no healing was observed in the wound-scratch assay. The PPP treatment induced cell apoptosis, as evidenced by enhanced caspase-3/7 activity; the proportion of annexin-V+/PI- cells was significantly higher in the HepG2 cells than in the non-hepatoma hepatocytes (16.4% +/- 0.4% vs. 5.8% +/- 0.2%, t = 14.05, P less than 0.01). After z-VAD-FMK treatment, the apoptosis rate was significantly higher in the HepG2 cells than in the non-hepatoma hepatocytes (11.3% +/- 0.7% vs. 5.8% +/- 0.2%, t = 11.83, P less than 0.01). IGF-IR is associated with proliferation, cell motility, and apoptosis of HCC cells, and may be a promising molecular target for HCC.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 052871560

Download citation: RISBibTeXText

Related references

Expression of lncRNA MSC-AS1 in hepatocellular carcinoma cell lines and its effect on proliferation, apoptosis, and migration. Turkish Journal of Gastroenterology: the Official Journal of Turkish Society of Gastroenterology 31(12): 860-867, 2020

Hsa-miR-7-5p suppresses proliferation, migration and promotes apoptosis in hepatocellular carcinoma cell lines by inhibiting SPC24 expression. Biochemical and Biophysical Research Communications 561: 80-87, 2021

Comparative Analysis of the Effects of 17-Beta Estradiol on Proliferation, and Apoptosis in Hepatocellular Carcinoma Hep G2 and LCL-Pi 11 Cell Lines. Asian Pacific Journal of Cancer Prevention: Apjcp 19(9): 2637-2641, 2018

Evaluation of the role of hexokinase type Ii in cellular proliferation and apoptosis using human hepatocellular carcinoma cell lines. Journal of Nuclear Medicine: Official Publication Society of Nuclear Medicine 50(9): 1525-1532, 2009

Arsenic trioxide induced apoptosis, inhibited cellular proliferation in human colon adenocarcinoma cell lines Its role in thymidylate synthase mRNA expression. Proceedings of the American Association for Cancer Research Annual Meeting 43: 212, 2002

Expression of Fas and anti-Fas-mediated apoptosis in human hepatocellular carcinoma cell lines. Journal of Hepatology 25(4): 454-464, 1996

Expression of Fas and Bcl-2 proteins and induction of apoptosis in human hepatocellular carcinoma cell lines. Medical Electron Microscopy: Official Journal of the Clinical Electron Microscopy Society of Japan 32(4): 199-203, 1999

Apoptosis and cell proliferation: correlation with BCL-2 and P53 oncoprotein expression in human hepatocellular carcinoma. Hepato-Gastroenterology 61(133): 1393-1401, 2014

Single-walled carbon nanohorn (SWNH) aggregates inhibited proliferation of human liver cell lines and promoted apoptosis, especially for hepatoma cell lines. International Journal of Nanomedicine 9: 759-773, 2014

Microvascular invasion in hepatocellular carcinoma overexpression promotes cell proliferation and inhibits cell apoptosis of hepatocellular carcinoma via inhibiting miR-199a expression. Oncotargets and Therapy 8: 2303-2310, 2015

The Blockage of KCa3.1 Channel Inhibited Proliferation, Migration and Promoted Apoptosis of Human Hepatocellular Carcinoma Cells. Journal of Cancer 6(7): 643-651, 2015

Effects of over-expression of ANXA10 gene on proliferation and apoptosis of hepatocellular carcinoma cell line HepG2. Journal of Huazhong University of Science and Technology. Medical Sciences 32(5): 669-674, 2012

Effects of human hepatocyte growth factor on the proliferation of human hepatocytes and hepatocellular carcinoma cell lines. European Surgical Research. Europaische Chirurgische Forschung. Recherches Chirurgicales Europeennes 36(5): 300-307, 2004

Effects of TNBG on the proliferation and apoptosis of human hepatocellular carcinoma cell line QGY-7701. Sichuan da Xue Xue Bao. Yi Xue Ban 36(3): 372-374, 2005

Effects of monoclonal antibodies against human stathmin 1 combined paclitaxel on proliferation of human hepatocellular carcinoma cell lines. Chinese-German Journal of Clinical Oncology 8(10): 603-606, 2009