+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Effects of interferons α/β on the proliferation of human micro- and macrovascular endothelial cells



Effects of interferons α/β on the proliferation of human micro- and macrovascular endothelial cells



Journal of Interferon and Cytokine Research 31(5): 451-458



Synthetic interferons (IFNs) are used in the treatment of several types of cancer. In addition to an antitumor effect, IFNs show antiangiogenic activity. The aim of this study was to investigate the effects of IFN-α and IFN-β on human micro- and macrovascular endothelial cells in vitro [human micro vascular lung endothelial cells (HMVEC-L) and human umbilical cord endothelial cells (HUVEC)]. By immunohistochemical staining and quantitative reverse transcriptase (RT)-polymerase chain reaction, we studied expression of type I IFN receptors. We evaluated the effects of IFN-α and IFN-β on the proliferation (DNA content), apoptosis (DNA fragmentation by enzyme-linked immunosorbent assay), and cell cycle distribution (flow-cytometric analysis) of endothelial cells. HUVEC and HMVEC-L cells show comparable expression level of the distinct IFN receptor subtypes. Proliferation of HMVEC-L and HUVEC was inhibited by IFN-β (the half maximal inhibitory concentration [IC(50)] = 60 and 90 IU/mL, respectively), but not by IFN-α at a dose up to 1,000 IU/mL. An interesting and unexpected observation was an inhibition of apoptosis by IFN-β. After 72 h of treatment with IFN-β. Cell cycle inhibition occurs in late S-phase in both cell lines. In conclusion, only IFN-β, not IFN-α (10-1,000 IU/mL), has an inhibitory activity on endothelial cell proliferation. Surprisingly, apoptosis was decreased by IFN treatment, whereas inhibition of proliferation is caused by cell cycle arrest in late S-phase.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052871982

Download citation: RISBibTeXText

PMID: 21361792

DOI: 10.1089/jir.2009.0103


Related references

Calcium-activated potassium channels and proliferation of micro- and macrovascular endothelial cells. Biophysical Journal 86(1): 348a, 2004

Selective blockade of the intermediate-conductance Ca2+-activated K+ channel supresses proliferation of micro- and macrovascular endothelial cells. FASEB Journal 18(4-5): Abst 169 7, 2004

Cultivation and characterization of micro- and macrovascular endothelial cells from the human heart. European Heart Journal 14 Suppl I: 74-81, 1993

Human Micro- and Macrovascular Endothelial Cells Exposed to Simulated Microgravity Upregulate hsp70. Microgravity Science and Technology 21(1-2): 141-144, 2009

Responses of micro- and macrovascular endothelial cells to hypoxia-reoxygenation. FASEB Journal 9(3): A36, 1995

Eicosanoid production by endothelial cells: a comparison between cells from micro- and macrovascular sources. Progress in Clinical and Biological Research 301: 371-375, 1989

Different effects of cytokines on human macrovascular and microvascular endothelial cells underline their phenotypic heterogeneity. Placenta 20(5-6): A 40, 1999

Micro- and macrovascular endothelial cells in beta-adrenergic regulation of transendothelial permeability. American Journal of Physiology 269(5 Pt 1): C1209-C1218, 1995

Functional Differences Between Placental Micro- and Macrovascular Endothelial Colony-Forming Cells. Stem Cells Translational Medicine 5(3): 291-300, 2016

The short prolactin receptor predominates in endothelial cells of micro- and macrovascular origin. Journal of Vascular Research 44(1): 19-30, 2007

Heterogeneity of microvascular endothelial cells isolated from human term placenta and macrovascular umbilical vein endothelial cells. European Journal of Cell Biology 82(4): 163-173, 2003

Release of CXC-chemokines by human lung microvascular endothelial cells (LMVEC) compared with macrovascular umbilical vein endothelial cells. Clinical and Experimental Immunology 118(2): 298-303, 1999

Homeobox genes are differentially expressed in macrovascular human umbilical vein endothelial cells and microvascular placental endothelial cells. Placenta 28(2-3): 219-223, 2006

Beta-adrenergic regulation of the permeability of cultured, micro- and macrovascular bovine endothelial cells. Biological Chemistry Hoppe-Seyler 375(SPEC Suppl. 1): S116, 1994

Human microvascular endothelial cells differ from macrovascular endothelial cells in their expression of matrix metalloproteinases. International Journal of Biochemistry and Cell Biology 29(10): 1167-1177, 1997