+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Effects of intra- and extracellular factors on anti-aging klotho gene expression



Effects of intra- and extracellular factors on anti-aging klotho gene expression



Genetics and Molecular Research 10(3): 2009-2023



Inactivation of the klotho gene in mice causes serious systemic disorders, resembling human aging. However, at the molecular level, its action mechanisms are not well understood. The stimulatory or inhibitory effects of cis- and trans-regulatory factors on the klotho gene expression are also still unclear. We studied the effects of intra- and extracellular factors on human klotho gene expression. For this purpose, pHKP-Luc and pHKP-GFP reporter vectors were constructed with the 2.1-kbp upstream region of human klotho, covering its promoter region, using luciferase and GFP genes as the reporter. A series of vectors that have deletions in the upstream region of the klotho gene were constructed to assay cis-acting factors. Deletion of some parts of the klotho gene upstream region significantly affected reporter gene expression in HEK293 cells. p16 and p53 proteins inhibited reporter luciferase expression under the control of human klotho promoter in a dose-dependent manner. Calcium and phosphate ions stimulated klotho expression. p21, PTH, IGF-1, and angiotensin-II had no significant effect on klotho expression in HEK293 cells.

(PDF emailed within 0-6 h: $19.90)

Accession: 052872184

Download citation: RISBibTeXText

PMID: 21948763

DOI: 10.4238/vol10-3gmr1261


Related references

Statin upregulates the expression of klotho, an anti-aging gene, in experimental cyclosporine nephropathy. Nephron. Experimental Nephrology 120(4): E123-E133, 2015

Vitamin D receptor controls expression of the anti-aging klotho gene in mouse and human renal cells. Biochemical and Biophysical Research Communications 414(3): 557-562, 2011

Testosterone increases renal anti-aging klotho gene expression via the androgen receptor-mediated pathway. Biochemical Journal 464(2): 221-229, 2015

Resveratrol increases anti-aging Klotho gene expression via the activating transcription factor 3/c-Jun complex-mediated signaling pathway. International Journal of Biochemistry & Cell Biology 53: 361-371, 2015

Studies on the anti-aging activity of a glycoprotein isolated from Fupenzi (Rubus chingii Hu.) and its regulation on klotho gene expression in mice kidney. International Journal of Biological Macromolecules, 2018

Regulation of S-formylglutathione hydrolase by the anti-aging gene klotho. Oncotarget 8(51): 88259-88275, 2017

Pulmonary emphysema in klotho mutant mice (deficient in klotho gene expression): klotho gene essential in postnatal pulmonary integrity. Nihon Kokyuki Gakkai Zasshi 40(3): 203-209, 2002

Characterization of the expression of the anti-aging protein Klotho in rodent and primate brain. 2007

Induction of anti-aging gene klotho with a small chemical compound that demethylates CpG islands. Oncotarget 8(29): 46745-46755, 2017

The anti-aging gene KLOTHO is a novel target for epigenetic silencing in human cervical carcinoma. Molecular Cancer 9(): 109-109, 2010

Activation of the Anti-Aging and Cognition-Enhancing Gene Klotho by CRISPR-dCas9 Transcriptional Effector Complex. Journal of Molecular Neuroscience, 2018

Deficiency in the anti-aging gene Klotho promotes aortic valve fibrosis through AMPK╬▒-mediated activation of RUNX2. Aging Cell 15(5): 853-860, 2016

Iron chelation and a free radical scavenger suppress angiotensin II-induced downregulation of klotho, an anti-aging gene, in rat. FEBS Letters 551(1-3): 58-62, 11 September, 2003

Genetic deficiency of anti-aging gene klotho exacerbates early nephropathy in STZ-induced diabetes in male mice. Endocrinology 154(10): 3855-3863, 2013

Klotho, an anti-aging gene, acts as a tumor suppressor and inhibitor of IGF-1R signaling in diffuse large B cell lymphoma. Journal of Hematology & Oncology 10(1): 37-37, 2017