+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Effects of small interfering RNAs targeting MAPK1 on gene expression profile in HeLa cells as revealed by microarray analysis

Effects of small interfering RNAs targeting MAPK1 on gene expression profile in HeLa cells as revealed by microarray analysis

Cell Biology International 32(9): 1081-1090

The mitogen activated protein kinases (MAPK) signaling cascade plays an important role in cell life. We proved that small interfering RNAs targeting MAPK1 (siRNA-2) could inhibit HeLa cell growth, but the effects of siRNA-2 on gene expression profile were unclear. Using Affymetrix GeneChip HG-U133A 2.0, we identified the long-term changes for 48 h in gene expression profile in HeLa cell treated by siRNA-2. The results showed that expressions of 181 genes were altered by siRNA-2 and were divided into two groups: (i) one group showed downregulation by siRNA-2, including the proliferation associated genes, small G protein, cytoskeleton associated protein and extracellular matrix associated protein; and (ii) the other group showed upregulation by siRNA-2, including interferon response genes, OAS family, TRIM family and apoptosis associated genes. The results of Real-time quantitative PCR for MAPK1, NUP188, P38, STAT1, STAT2, MPL and OAS1 were consistent with that of gene chip. Two networks were found to react substantially to the downregulation of MAPK1 by siRNA-2. One of the networks regulates cell growth through cell-cycle control, apoptosis and cytoskeleton. The other network is related to interferon-like response. Our findings suggest that siRNA-mediated downregulation of MAPK1 could be an attractive strategy for cancer therapy.

(PDF emailed within 0-6 h: $19.90)

Accession: 052886084

Download citation: RISBibTeXText

PMID: 18539490

DOI: 10.1016/j.cellbi.2008.04.019

Related references

Effects of small interfering RNAs targeting Fascin on gene expression in oral cancer cells. Journal of Oral Pathology & Medicine 38(9): 722-730, 2009

Treatment of HeLa cells with small interfering RNAs against HuR or Lamin A and C results in cyclooxygenase-2 expression. European Cytokine Network 14(Supplement 3): 39, Sept, 2003

Inhibition of hepatitis B virus gene expression by small interfering RNAs targeting cccDNA and X antigen. Acta Virologica 56(1): 49-55, 2012

Resistance to Ditylenchus destructor Infection in Sweet Potato by the Expression of Small Interfering RNAs Targeting unc-15, a Movement-Related Gene. Phytopathology 105(11): 1458-1465, 2016

Effects of U0126 and fibroblast growth factor on gene expression profile in Ciona intestinalis embryos as revealed by microarray analysis. Development, Growth & Differentiation 48(6): 391-400, 2006

Screening effective sequences of small interfering RNAs targeting MDR1 gene in human gastric cancer SGC7901/VCR cells. Zhonghua Zhong Liu Za Zhi 28(3): 178-182, 2006

Silencing of gene expression in cultured cells using small interfering RNAs. Current Protocols in Cell Biology Chapter 27: Unit 27.1.1-28, 2010

Effective small interfering RNAs targeting matrix and nucleocapsid protein gene inhibit influenza A virus replication in cells and mice. Antiviral Research 76(2): 186-193, 2007

Gene microarray analysis of the circular RNAs expression profile in human gastric cancer. Oncology Letters 15(6): 9965-9972, 2018

Inhibition of porcine circovirus type 1 and type 2 production in PK-15 cells by small interfering RNAs targeting the Rep gene. Veterinary Microbiology 123(1-3): 203-209, 2007

Analysis of gene function in somatic mammalian cells using small interfering RNAs. Methods 26(2): 199-213, 2002

Small interfering RNAs targeting the rabies virus nucleoprotein gene. Virus Research 169(1): 169-174, 2013

Modulation of MDR1 gene expression in multidrug resistant MCF7 cells by low concentrations of small interfering RNAs. Biochemical Pharmacology 70(10): 1424-1430, 2005

Transcriptional targeting of small interfering RNAs into cancer cells. Biochemical and Biophysical Research Communications 350(4): 854-859, 2006

Selective silencing of viral gene E6 and E7 expression in HPV-positive human cervical carcinoma cells using small interfering RNAs. Methods in Molecular Biology 292: 401-420, 2004