+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Endothelial progenitor cells promote directional three-dimensional endothelial network formation by secreting vascular endothelial growth factor



Endothelial progenitor cells promote directional three-dimensional endothelial network formation by secreting vascular endothelial growth factor



Plos one 8(12): E82085



Endothelial progenitor cell (EPC) transplantation induces the formation of new blood-vessel networks to supply nutrients and oxygen, and is feasible for the treatment of ischemia and cardiovascular diseases. However, the role of EPCs as a source of proangiogenic cytokines and consequent generators of an extracellular growth factor microenvironment in three-dimensional (3D) microvessel formation is not fully understood. We focused on the contribution of EPCs as a source of proangiogenic cytokines on 3D microvessel formation using an in vitro 3D network model. To create a 3D network model, EPCs isolated from rat bone marrow were sandwiched with double layers of collagen gel. Endothelial cells (ECs) were then cultured on top of the upper collagen gel layer. Quantitative analyses of EC network formation revealed that the length, number, and depth of the EC networks were significantly enhanced in a 3D model with ECs and EPCs compared to an EC monoculture. In addition, conditioned medium (CM) from the 3D model with ECs and EPCs promoted network formation compared to CM from an EC monoculture. We also confirmed that EPCs secreted vascular endothelial growth factor (VEGF). However, networks cultured with the CM were shallow and did not penetrate the collagen gel in great depth. Therefore, we conclude that EPCs contribute to 3D network formation at least through indirect incorporation by generating a local VEGF gradient. These results suggest that the location of EPCs is important for controlling directional 3D network formation in the field of tissue engineering.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 052971405

Download citation: RISBibTeXText

PMID: 24312630


Related references

Angiotensin Ii Potentiates Vascular Endothelial Growth Factor-Induced Proliferation and Network Formation of Endothelial Progenitor Cells. Hypertension Research 27(2): 101-108, 2004

Angiotensin II potentiates vascular endothelial growth factor-induced proliferation and network formation of endothelial progenitor cells. Hypertension Research 27(2): 101-108, 2004

Hydrodynamic shear-stress-dependent retention of endothelial and endothelial progenitor cells adhered to vascular endothelial growth factor-fixed surfaces. Journal of Biomedical Materials Research. Part B Applied Biomaterials 100(5): 1218-1228, 2012

Afadin Facilitates Vascular Endothelial Growth Factor-Induced Network Formation and Migration of Vascular Endothelial Cells by Inactivating Rho-Associated Kinase Through ArhGAP29. Arteriosclerosis Thrombosis and Vascular Biology 38(5): 1159-1169, 2018

Gene transfers of vascular endothelial growth factor-A, vascular endothelial growth factor-B, vascular endothelial growth factor-C, and vascular endothelial growth factor-D have no effects on atherosclerosis in hypercholesterolemic low-density lipoprotein-receptor/apolipoprotein B48-deficient mice. Circulation 112(9): 1347-1352, 2005

Proliferation hemangiomas formation through dual mechanism of vascular endothelial growth factor mediated endothelial progenitor cells proliferation and mobilization through matrix metalloproteinases 9. Medical Hypotheses 70(4): 815-818, 2008

Mesenchymal stem cells promote endothelial progenitor cell proliferation by secreting insulin‑like growth factor‑1. Molecular Medicine Reports 16(2): 1502-1508, 2017

Quantification of circulating vascular endothelial growth factor receptor-3-positive lymphatic/vascular endothelial progenitor cells. Clinical Cancer Research 15(21): 6740; Author Reply 6740, 2009

Transplantation of endothelial progenitor cells transferred by vascular endothelial growth factor gene for vascular regeneration of ischemic flaps. Journal of Surgical Research 135(1): 100-106, 2006

Relationship between endothelial progenitor cells and vascular endothelial growth factor and its variation with exercise. Thrombosis Research 137: 92-96, 2016

Endothelial progenitor cells and vascular endothelial growth factor in patients with Takayasu's arteritis. Advances in Rheumatology 58(1): 24, 2018

Vascular endothelial growth factor-stimulated endothelial cells promote adhesion and activation of platelets. Blood 96(13): 4216-4221, 2000

Endostatin inhibits the vascular endothelial growth factor-induced mobilization of endothelial progenitor cells. Cancer Research 63(23): 8345-8350, 2003

Chimeric vascular endothelial growth factor fusion protein selectively inhibits Flk-1 endothelial progenitor cells. Journal of the American College of Cardiology 39(5 Suppl. A): 204A, 2002

Tissue engineering of bladder using vascular endothelial growth factor gene-modified endothelial progenitor cells. International Journal of Artificial Organs 34(12): 1137-1146, 2011