+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Enhanced arsenic accumulation in Saccharomyces cerevisiae overexpressing transporters Fps1p or Hxt7p

Enhanced arsenic accumulation in Saccharomyces cerevisiae overexpressing transporters Fps1p or Hxt7p

Journal of Biotechnology 150(1): 101-107

Arsenic contamination of ground water affects the health of millions of people worldwide. Bioremediation has the potential to lower contaminant levels in cases where physical methods are either ineffective or cost prohibitive. The yeast Saccharomyces cerevisiae was engineered for enhanced arsenite accumulation by overexpression of transporters responsible for the influx of the contaminant. The transporter genes FPS1 and HXT7 were cloned under the control of the late-phase ADH2-promoter. This allowed for protein production at high biomass levels without the addition of inducer. Following the transfer of stationary phase cells to buffer, the engineered strains were capable of 3-4-fold greater arsenic uptake as compared to control cells. Further, at trace levels of the metalloid, the cells overexpressing the Fps1p transporter removed ca. 40% more arsenite from the extracellular medium than the controls. Arsenic uptake was also evaluated in cells overexpressing the transporters coupled with high-level production of cytosolic As sequestors (phytochelatins or bacterial ArsRp) to act as an intracellular sink. This led to an up to 4-fold increase in As accumulation in the resting cell culture as compared to native cells. The results demonstrate important steps needed to engineer a yeast biosorbent with enhanced accumulation capabilities for this metalloid.

(PDF emailed within 0-6 h: $19.90)

Accession: 052982204

Download citation: RISBibTeXText

PMID: 20638426

DOI: 10.1016/j.jbiotec.2010.07.012

Related references

Improvement of glucose uptake rate and production of target chemicals by overexpressing hexose transporters and transcriptional activator Gcr1 in Saccharomyces cerevisiae. Applied and Environmental Microbiology 81(24): 8392-8401, 2016

Components of the Vid30c are needed for the rapamycin-induced degradation of the high-affinity hexose transporter Hxt7p in Saccharomyces cerevisiae. Fems Yeast Research 8(2): 204-216, 2007

Enhanced arsenate uptake in Saccharomyces cerevisiae overexpressing the Pho84 phosphate transporter. Biotechnology Progress 28(3): 654-661, 2012

The glycerol channel Fps1p mediates the uptake of arsenite and antimonite in Saccharomyces cerevisiae. Molecular Microbiology 40(6): 1391-1401, June, 2001

Proprietes et controle genetiques du systeme d-accumulation des acides amines chez Saccharomyces cerevisiae Properties and genetic control of the amino acid accumulation system in Saccharomyces cerevisiae. Biochim Biophys Acta: 546-566, 1965

Deletion of FPS1, encoding aquaglyceroporin Fps1p, improves xylose fermentation by engineered Saccharomyces cerevisiae. Applied and Environmental Microbiology 79(10): 3193-3201, 2013

Increased ethanol accumulation from glucose via reduction of ATP level in a recombinant strain of Saccharomyces cerevisiae overexpressing alkaline phosphatase. Bmc Biotechnology 14: 42, 2015

Transgenic tomato overexpressing ath-miR399d has enhanced phosphorus accumulation through increased acid phosphatase and proton secretion as well as phosphate transporters. Plant and Soil 334(1-2): 123-136, 2010

Enhanced tolerance to and accumulation of mercury, but not arsenic, in plants overexpressing two enzymes required for thiol peptide synthesis. Physiologia plantarum 128(1): 48-57, 2006

Fps1p channel is the mediator of the major part of glycerol passive diffusion in Saccharomyces cerevisiae: Artefacts and re-definitions. Biochimica et Biophysica Acta 1613(1-2): 57-71, 27 June, 2003

Transgenic tobacco plants overexpressing the Met25 gene of Saccharomyces cerevisiae exhibit enhanced levels of cysteine and glutathione and increased tolerance to oxidative stress. Amino Acids 30(2): 185-194, 2005

Enhanced S-adenosyl-l-methionine production in Saccharomyces cerevisiae by spaceflight culture, overexpressing methionine adenosyltransferase and optimizing cultivation. Journal of Applied Microbiology 112(4): 683-694, 2012

Zinc status and vacuolar zinc transporters control alkaline phosphatase accumulation and activity in Saccharomyces cerevisiae. Molecular Microbiology 72(2): 320-334, 2009

Comparative proteomic analysis of engineered Saccharomyces cerevisiae with enhanced free fatty acid accumulation. Applied Microbiology and Biotechnology 100(3): 1407-1420, 2016

Structural and functional conservation between the high-affinity K+ transporters of Saccharomyces uvarum and Saccharomyces cerevisiae. Gene 99(1): 39-46, 1991