+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Enhanced bone morphogenic protein adenoviral gene delivery to bone marrow stromal cells using magnetic nanoparticle



Enhanced bone morphogenic protein adenoviral gene delivery to bone marrow stromal cells using magnetic nanoparticle



Journal of the Korean Association of Oral and Maxillofacial Surgeons 39(3): 112-119



This study investigated the question of whether adenoviral magnetofection can be a suitable method for increasing the efficacy of gene delivery into bone marrow stromal cell (BMSC) and for generation of a high level of bone morphogenic protein (BMP) secretion at a minimized viral titer. Primary BMSCs were isolated from C57BL6 mice and transduced with adenoviral vectors encoding β galactosidase or BMP2 and BMP7. The level of BMP secretion, activity of osteoblast differentiation, and cell viability of magnetofection were measured and compared with those of the control group. The expression level of β galactosidase showed that the cell transduction efficiency of AdLacZ increased according to the increased amount of magnetic nanoparticles. No change in cell viability was observed after magnetofection with 2 µL of magnetic nanoparticle. Secretion of BMP2 or BMP7 was accelerated after transduction of AdBMP2 and 7 with magnetofection. AdBMP2 adenoviral magnetofection resulted in up to 7.2-fold higher secretion of BMP2, compared with conventional AdBMP2-transduced BMSCs. Magnetofection also induced a dramatic increase in secretion of BMP7 by up to 10-fold compared to the control. Use of only 1 multiplicity of infection (moi) of magnetofection with adenoviral transduction of AdBMP2 or AdBMP7 resulted in significantly higher transgene expression compared to 20 moi of conventional adenoviral transduction. Magnetic particle-mediated gene transudation is a highly efficient method of gene delivery to BMSCs. Magnetofection can lower the amount of viral particles while improving the efficacy of gene delivery.

(PDF emailed within 0-6 h: $19.90)

Accession: 052982474

Download citation: RISBibTeXText

PMID: 24471028

DOI: 10.5125/jkaoms.2013.39.3.112


Related references

Large-scale bicortical skull bone regeneration using ex vivo replication-defective adenoviral-mediated bone morphogenetic protein-2 gene-transferred bone marrow stromal cells and composite biomaterials. Neurosurgery 65(6 Suppl): 75-81; Discussion 81-3, 2010

Effects of recombinant human bone morphogenic protein-2 and human bone marrow-derived stromal cells on in vivo bone regeneration of chitosan-poly(ethylene oxide) hydrogel. Journal of Biomedical Materials Research. Part A 101(3): 892-901, 2013

Enhanced hematopoietic recovery after coinfusion of hematopoietic stem cells and bone marrow stromal cells which were transfected with adenoviral vector carrying the GM-CSF gene in a mouse BMT model. Blood 96(11 Part 2): 387b, November 16, 2000

In vitro osteogenic induction of bone marrow stromal cells with encapsulated gene-modified bone marrow stromal cells and in vivo implantation for orbital bone repair. Tissue Engineering. Part A 20(13-14): 2019-2029, 2015

In vitro response of primary human bone marrow stromal cells to recombinant human bone morphogenic protein-2 in the early and late stages of osteoblast differentiation. Development, Growth & Differentiation 50(7): 553-564, 2009

Enhanced in vitro mineralization of primary bone marrow stromal cells following Runx2/Cbfa1 overexpression via retroviral gene delivery. Journal of Bone & Mineral Research 17(Suppl 1): S440, September, 2002

Bone morphogenic protein antagonists are coexpressed with bone morphogenic protein 4 in endothelial cells exposed to unstable flow in vitro in mouse aortas and in human coronary arteries: role of bone morphogenic protein antagonists in inflammation and atherosclerosis. Circulation 116(11): 1258-1266, 2007

Controlled differentiation of human bone marrow stromal cells using magnetic nanoparticle technology. Tissue Engineering. Part A 16(10): 3241-3250, 2011

Effective cytokine gene transfer to bone marrow stromal cells using adenoviral vectors. Journal of Cellular Biochemistry Supplement 0(21A): 406, 1995

Bone formation in rabbit's leg muscle after autologous transplantation of bone marrow-derived mesenchymal stem cells expressing human bone morphogenic protein-2. Indian Journal of Orthopaedics 48(4): 347-353, 2014

In vitro differentiation of bone marrow stromal cells into neurons and glial cells and differential protein expression in a two-compartment bone marrow stromal cell/neuron co-culture system. Journal of Clinical Neuroscience 17(7): 908-913, 2010

Dual delivery of rhPDGF-BB and bone marrow mesenchymal stromal cells expressing the BMP2 gene enhance bone formation in a critical-sized defect model. Tissue Engineering. Part A 19(21-22): 2495-2505, 2014

Adenoviral Mediated Expression of BMP2 by Bone Marrow Stromal Cells Cultured in 3D Copolymer Scaffolds Enhances Bone Formation. Plos One 11(1): E0147507-E0147507, 2016

Adenoviral-based gene transfer of two forms of stem cell factor to human bone marrow stromal cells. Blood 90(10 SUPPL 1 PART 2): 413B, Nov 15, 1997

The use of bone marrow stromal cells (bone marrow-derived multipotent mesenchymal stromal cells) for alveolar bone tissue engineering: basic science to clinical translation. Tissue Engineering. Part B, Reviews 20(3): 229-232, 2015