+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Enhanced cell affinity of chitosan membranes mediated by superficial cross-linking: a straightforward method attainable by standard laboratory procedures



Enhanced cell affinity of chitosan membranes mediated by superficial cross-linking: a straightforward method attainable by standard laboratory procedures



Biomacromolecules 15(1): 291-301



It is well accepted that the surface modification of biomaterials can improve their biocompatibility. In this context, techniques like ion etching, plasma-mediated chemical functionalization, electrospinning, and contact microprinting have successfully been employed to promote the cell adhesion and proliferation of chitosan (CH) substrates. However, they prove to be time-consuming, highly dependent on environmental conditions, and/or limited to the use of expensive materials and sophisticated instruments not accessible to standard laboratories, hindering to a high extent their straightforward application. Filling this gap, this paper proposes the superficial cross-linking of CH as a much simpler and accessible means to modify its superficial properties in order to enhance its cellular affinity. CH membranes were prepared by solvent casting followed by a cross-linking step mediated by the chemical vapor deposition (CVD) of glutaraldehyde (GA). The membranes were characterized against non- and solution cross-linked membranes in terms of their mechanical/surface properties and biological performance. Among others, the CVD membranes proved (i) to be more mechanically stable against cell culture and sterilization than membranes cross-linked in solution and (ii) to prompt the adherence and sustained proliferation of healthy cells to levels even superior to commercial tissue culture plates (TCPs). Accordingly, the CVD cross-linking approach was demonstrated to be a simple and cost-effective alternative to the aforementioned conventional methods. Interestingly, this concept can also be applied to other biomaterials as long as GA (or other volatile components alike) can be employed as a cross-linker, making possible the cross-linking reaction at mild experimental conditions, neither requiring sophisticated lab implements nor using any potentially harmful procedure.

(PDF emailed within 0-6 h: $19.90)

Accession: 052982632

Download citation: RISBibTeXText

PMID: 24328099

DOI: 10.1021/bm401541v


Related references

Photochemical cross-linking of cell membranes. A test for natural and random collisional cross-links by millisecond cross-linking. Journal of Biological Chemistry 252(23): 8524-8531, 1977

Affinity cross-linking of bradykinin B-2 receptors in guinea pig ileum membranes. European Journal Of Pharmacology Molecular Pharmacology Section. 16(2): 271-274, 1994

Transport of small anionic and neutral solutes through chitosan membranes: dependence on cross-linking and chelation of divalent cations. Biomacromolecules 9(8): 2132-2138, 2008

Standard versus trans-epithelial collagen cross-linking in keratoconus patients suitable for standard collagen cross-linking. Clinical Ophthalmology 9(): 503-509, 2015

Effects of porogen and cross-linking agents on improved properties of silica-supported macroporous chitosan membranes for enzyme immobilization. Journal of Membrane Biology 248(2): 231-240, 2015

Initiation factor if 3 cross linking to escherichia coli ribosomal 30s subunits by 3 different light dependent procedures identification of 30s proteins cross linked to if 3 utilization of a new 2 stage cross linking reagent p nitrobenzyl maleimide. Archives of Biochemistry & Biophysics 208(2): 554-562, 1981

Identification of a receptor for atrial natriuretic factor in rabbit aorta membranes by affinity cross-linking. Journal of Biological Chemistry 260(20): 10889-10892, 1985

Identification of the calmodulin binding proteins in rat adipocyte plasma membranes by photo affinity cross linking. Federation Proceedings 41(3): ABSTRACT 1372, 1982

Development of enhanced capacity affinity microcolumns by using a hybrid of protein cross-linking/modification and immobilization. Journal of Chromatography. A 1400: 82-90, 2015

Affinity directed cross linking of acetyl choline receptor poly peptide components in postsynaptic membranes. Biochemical and Biophysical Research Communications 85(2): 623-631, 1978

Factor XIIIa-mediated cross-linking of fibronectin in fibroblast cell layers. Cross-linking of cellular and plasma fibronectin and of amino-terminal fibronectin fragments. Journal of Biological Chemistry 264(7): 4179-4185, 1989

Reconstitution of high cell binding affinity of a marine sponge aggregation factor by cross-linking of small low affinity fragments into a large polyvalent polymer. Journal of Biological Chemistry 261(6): 2853-2859, 1986

Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase. Food and Bioproducts Processing, 2013

Light-enhanced cross-linking of rhodopsin in rod outer segment membranes as detected by chemical probes. Biochimica et Biophysica Acta 603(2): 313-321, 1980