+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Enhanced cerebral ischemic lesions after two-vein occlusion in diabetic rats



Enhanced cerebral ischemic lesions after two-vein occlusion in diabetic rats



Brain Research 1309: 126-135



The two-vein occlusion (2VO) model is known to be useful for ischemic penumbra studies in vivo. It was applied here to examine sequential changes of regional cerebral blood flow (CBF) and cerebral venous infarction in normal (Long-Evans Tokushima Otsuka, LETO) and diabetic (Otsuka Long-Evans Tokushima Fatty, OLETF) rats. The aim of our study was to examine and compare the ischemic pathogenesis related to regional changes in cerebral blood flow (CBF) induced with 2VO in diabetic OLETF and non-diabetic LETO rats. Two cortical veins were occluded photochemically by using rose bengal dye in 10 OLETF and 10 LETO rats. All animals were killed with perfusion fixation at 48 h after 2VO. Bax and Bcl-2 staining was performed along with the terminal deoxynucleotidyl transderase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay to examine the relationship to single-cell death. Smooth muscle actin and Van Gieson's elastic staining were done for assessment of thickening of the vessel walls. Not only the volume of cerebral cortex affected by 2VO-induced venous infarction was increased in diabetic OLETF rats, but we also observed significantly reduced CBF at 90 min after 2VO, coupled with increased apoptosis in and around ischemic lesions. Morphologically, OLETF rats demonstrated marked thickening of the walls in the small cerebral vessels with perivascular fibrosis, indicating more severe cerebral microvascular atherosclerotic changes as compared to their non-diabetic LETO counterparts. The OLETF rat thus appears to be an excellent animal model for studying the diabetic enhancement of venous ischemia induced by 2VO.

(PDF emailed within 0-6 h: $19.90)

Accession: 052982715

Download citation: RISBibTeXText

PMID: 19896474

DOI: 10.1016/j.brainres.2009.10.061


Related references

Recurrent Hypoglycemia Exacerbates Cerebral Ischemic Damage in Diabetic Rats via Enhanced Post-Ischemic Mitochondrial Dysfunction. Translational Stroke Research, 2018

Ischemic cortical lesions after permanent occlusion of individual middle cerebral artery branches in rats. Stroke 19(7): 870-877, 1988

Acute pre-ischemic normalization of plasma glucose in diabetic rats worsens neurologic outcomes following transient middle cerebral artery occlusion. Anesthesiology (Hagerstown) 87(3 SUPPL ): A716, 1997

Cerebral aspergillosis in a diabetic patient leading to cerebral artery occlusion and ischemic stroke: a case report and literature review. Journal of Stroke and Cerebrovascular Diseases 24(1): E39-E43, 2015

Focal cerebral ischemic model using middle cerebral artery occlusion in ovariectomized rats. Society for Neuroscience Abstracts 22(1-3): 1668, 1996

Cerebral blood flow changes at ischemic penumbra before and after middle cerebral artery occlusion in the rats. Neurosciences 21(SUPPL ): P35-P38, 1995

Suppressive effect of E-64c on ischemic degradation of cerebral proteins following occlusion of the middle cerebral artery in rats. Brain Research 526(1): 177-179, 1990

Protective effects of sophora subprostrata against focal cerebral ischemic damage by middle cerebral artery occlusion in rats. Society for Neuroscience Abstracts 26(1-2): Abstract No -287 15, 2000

High sodium chloride diet predisposes rats to cerebral ischemic infarction after middle cerebral artery mca occlusion. Federation Proceedings 46(3): 1072, 1987

Coronary artery disease, cerebral non-fatal ischemic stroke in retinal vein occlusion: an 8-yr follow-up. Nutrition, Metabolism, and Cardiovascular Diseases 22(1): 23-27, 2012

Beneficial effect of prolonged administration of albumin on ischemic cerebral edema and infarction after occlusion of middle cerebral artery in rats. Neurosurgery 33(2): 293-300; Comment 300, 1993

Post-treatment with aminoguanidine reduces cerebral ischemic damage and neurological deficits in rats with middle cerebral artery occlusion. Stroke 29(1): 331, 1998

Temporal profile of astrocytes and changes of oligodendrocyte-based myelin following middle cerebral artery occlusion in diabetic and non-diabetic rats. International Journal of Biological Sciences 9(2): 190-199, 2013

Protective Effect of Guggulipid in High Fat Diet and Middle Cerebral Artery Occlusion (MCAO) Induced Ischemic Cerebral Injury in Rats. Drug Research 66(8): 407-414, 2016

Cerebral Blood Flow and Glucose Metabolism of the Ischemic Rim in Spontaneously Hypertensive Stroke-Prone Rats with Occlusion of the Middle Cerebral Artery. Journal Of Cerebral Blood Flow & Metabolism. 15(2): 235-241, 1995