+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Enhanced co-production of hydrogen and poly-(R)-3-hydroxybutyrate by recombinant PHB producing E. coli over-expressing hydrogenase 3 and acetyl-CoA synthetase

Enhanced co-production of hydrogen and poly-(R)-3-hydroxybutyrate by recombinant PHB producing E. coli over-expressing hydrogenase 3 and acetyl-CoA synthetase

Metabolic Engineering 14(5): 496-503

Recombinant Escherichia coli was constructed for co-production of hydrogen and polyhydroxybutyrate (PHB) due to its rapid growth and convenience of genetic manipulation. In particular, anaerobic metabolic pathways dedicated to co-production of hydrogen and PHB were established due to the advantages of directing fluxes away from toxic compounds such as formate and acetate to useful products. Here, recombinant E. coli expressing hydrogenase 3 and/or acetyl-CoA synthetase showed improved PHB and hydrogen production when grown with or without acetate as a carbon source. When hydrogenase 3 was over-expressed, hydrogen yield was increased from 14 to 153 mmol H(2)/mol glucose in a mineral salt (MS) medium with glucose as carbon source, accompanied by an increased PHB yield from 0.55 to 5.34 mg PHB/g glucose in MS medium with glucose and acetate as carbon source.

(PDF emailed within 0-6 h: $19.90)

Accession: 052982829

Download citation: RISBibTeXText

PMID: 22842473

DOI: 10.1016/j.ymben.2012.07.003

Related references

Enhanced production of poly-3-hydroxybutyrate by Escherichia coli over-expressing multiple copies of NAD kinase integrated in the host genome. Biotechnology Letters 37(6): 1273-1278, 2016

Enhanced production of poly‑3‑hydroxybutyrate (PHB) by expression of response regulator DR1558 in recombinant Escherichia coli. International Journal of Biological Macromolecules 2019, 2019

Enhanced production of poly(3-hydroxybutyrate) by filamentation-suppressed recombinant Escherichia coli in a defined medium. Journal of Environmental Polymer Degradation 4(2): 131-134, 1996

Enhanced production of poly(3-hydroxybutyrate) in recombinant Escherichia coli and EDTA-microwave-assisted cell lysis for polymer recovery. Amb Express 8(1): 142, 2018

Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in recombinant Escherichia coli grown on glucose. Journal of Biotechnology 58(1): 33-38, 1997

Engineering Escherichia coli for enhanced production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in larger cellular space. Metabolic Engineering 25: 183-193, 2015

Application of a propionyl coenzyme A synthetase for poly(3-hydroxypropionate-co-3-hydroxybutyrate) accumulation in recombinant Escherichia coli. Applied and Environmental Microbiology 66(12): 5253-5258, 2000

Microbial production of 4-hydroxybutyrate, poly-4-hydroxybutyrate, and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by recombinant microorganisms. Applied Microbiology and Biotechnology 84(5): 909-916, 2009

Poly(3-hydroxybutyrate) production from whey using recombinant Escherichia coli. Biotechnology Letters 19(10): 1033-1035, 1997

Poly(3-hydroxybutyrate) production from xylose by recombinant Escherichia coli. Bioprocess Engineering 18(5): 397-399, 1998

Production of poly-β-hydroxybutyrate on molasses by recombinant Escherichia coli. Biotechnology Letters 20(4): 345-348, 1998

Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) by recombinant Escherichia coli expressing leucine metabolism-related enzymes derived from Clostridium difficile. Journal of Bioscience and Bioengineering 117(6): 670-675, 2014

Enhanced Hydrogen Production by Co-cultures of Hydrogenase and Nitrogenase in Escherichia coli. Current Microbiology 72(3): 242-247, 2016

Metabolic analysis of poly(3-hydroxybutyrate) production by recombinant Escherichia coli. Journal of Microbiology and Biotechnology 9(5): 593-603, 1999

Production of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) in a recombinant Escherichia coli strain. Applied and Environmental Microbiology 58(4): 1089-1094, 1992