EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Enrichment and purification of gardenia yellow from Gardenia jasminoides var. radicans Makino by column chromatography technique


Enrichment and purification of gardenia yellow from Gardenia jasminoides var. radicans Makino by column chromatography technique



Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 893-894: 43-48



ISSN/ISBN: 1570-0232

PMID: 22436821

DOI: 10.1016/j.jchromb.2012.02.032

In present study, the performance and separation characteristics of nine macroporous resins for the enrichment and purification of gardenia yellow from Gardenia jasminoides var. radicans Makino have been evaluated. The adsorption and desorption properties of crude gardenia yellow solution on macroporous resins including HPD722, HPD100, HPD100A, HPD400, HPD400A, D101, AB-8, XAD-16, and NKA-9 have been compared. Then, HPD722 was chosen to purify gardenia yellow because of its strong adsorption and desorption abilities as well as high selectivity. Column packed with HPD722 resin was used to perform dynamic adsorption and desorption tests to optimize the separation process of gardenia yellow. The optimal conditions were as follows: The crude gardenia yellow solution with concentration of 15 mg/mL was loaded in column packed with HPD722 resin at the flow rate of 1.0 mL/min, and the adsorbate-laden column was washed with 800 mL water, 600 mL 15% ethanol water solution respectively at the speed of 2.5 mL/min, then desorbed with 200 mL 80% ethanol water solution at the speed of 3.5 mL/min. The colority of the product obtained were up to 300. The method developed in this study provides a new approach for scale-up separation and purification of gardenia yellow from G. jasminoides var. radicans Makino.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052993235

Download citation: RISBibTeXText

Related references

A new triterpenoid from the fruits of Gardenia jasminoides var. radicans Makino. Natural Product Research 29(7): 633-637, 2015

Phytochemical compounds from the underground parts of Gardenia jasminoides var. radicans Makino. Korean Journal of Pharmacognosy 33(1): 1-4, 2002

Extraction optimization by using response surface methodology and purification of yellow pigment from Gardenia jasminoides var. radicans Makikno. Food Science and Nutrition 9(2): 822-832, 2021

Natural yellow colors extracted from gardenia fruit gardenia jasminoides and colors found in commercial gardenia fruit extract color analysis of natural yellow colors by high performance liquid chromatography studies on the quality of natural coloring matters 2. Journal of the Food Hygienic Society of Japan 26(2): 150-159, 1985

Gardenia poisoning in pigs. I. Reproduction of a Black discoloration of the viscera in animals with the seed-pods of Gardenia jasminoides Ellis. f. grandiflora Makino. Journal of the Chinese Society of Veterinary Science 2(1): 26-29, 1976

Selective Extraction of Gardenia Yellow and Geniposide from Gardenia jasminoides by Mechanochemistry. Molecules 21(5), 2016

Inhibitory Effects of Gardenia jasminoides var. radicans Makino on HIV-1 Enzymes and Prediction of Inhibitory Factor by QSAR. Korean Journal of Plant Reources. 27(1): 22-28, 2014

Geniposide from Gardenia jasminoides var. radicans Makino Attenuates Myocardial Injury in Spontaneously Hypertensive Rats via Regulating Apoptotic and Energy Metabolism Signalling Pathway. Drug Design Development and Therapy 15: 949-962, 2021

Food Chemical Properties of Crocetin Derivatives in Gardenia(Gardenia jasminoides Ellis) Yellow Color. Nippon Shokuhin Kagaku Kogaku Kaishi 42(10): 784-789, 1995

Comparison of natural yellow colors extracted from saffron crocus sativus and gardenia fruit gardenia jasminoides. Bulletin of National Institute of Hygienic Sciences (Tokyo) (103): 157-160, 1985

Homogenate extraction of gardenia yellow pigment from Gardenia Jasminoides Ellis fruit using response surface methodology. Journal of Food Science and Technology 51(8): 1575-1581, 2014

Comparison of natural yellow colors extracted from saffron (Crocus sativus Linne) and gardenia fruit (Gardenia jasminoides Ellis). Eisei Shikenjo Hokoku. Bulletin of National Institute of Hygienic Sciences 103: 157-160, 1985

Genetic relationships between Gardenia jasminoides var. radicans and G. jasminoides for. grandiflora revealed by randomly amplified polymorphic DNA. Journal of the Korean Society for Horticultural Science 46(1): 69-75, 2005

Essential Oils of Gardenia jasminoides J. Ellis and Gardenia jasminoides f. longicarpa Z.W. Xie and M. Okada Flowers: Chemical Characterization and Assessment of Anti-Inflammatory Effects in Alveolar Macrophage. Pharmaceutics 14(5), 2022

Isolation and purification of iridoid glycosides from Gardenia jasminoides Ellis by isocratic reversed-phase two-dimensional preparative high-performance liquid chromatography with column switch technology. Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 858(1-2): 296-301, 2007