+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Ensembles of engineered cardiac tissues for physiological and pharmacological study: heart on a chip

Ensembles of engineered cardiac tissues for physiological and pharmacological study: heart on a chip

Lab on a Chip 11(24): 4165-4173

Traditionally, muscle physiology experiments require multiple tissue samples to obtain morphometric, electrophysiological, and contractility data. Furthermore, these experiments are commonly completed one at a time on cover slips of single cells, isotropic monolayers, or in isolated muscle strips. In all of these cases, variability of the samples hinders quantitative comparisons among experimental groups. Here, we report the design of a "heart on a chip" that exploits muscular thin film technology--biohybrid constructs of an engineered, anisotropic ventricular myocardium on an elastomeric thin film--to measure contractility, combined with a quantification of action potential propagation, and cytoskeletal architecture in multiple tissues in the same experiment. We report techniques for real-time data collection and analysis during pharmacological intervention. The chip is an efficient means of measuring structure-function relationships in constructs that replicate the hierarchical tissue architectures of laminar cardiac muscle.

(PDF emailed within 0-6 h: $19.90)

Accession: 052993777

Download citation: RISBibTeXText

PMID: 22072288

DOI: 10.1039/c1lc20557a

Related references

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy. Journal of Visualized Experiments 2016(109): E53447, 2016

Oriented and Vectorial Patterning of Cardiac Myocytes Using a Microfluidic Dielectrophoresis Chip—Towards Engineered Cardiac Tissue With Controlled Macroscopic Anisotropy. Journal of Microelectromechanical Systems 15(6): 1483-1491, 2006

Organs-on-a-Chip: A Fast Track for Engineered Human Tissues in Drug Development. Cell Stem Cell 22(3): 310-324, 2018

Determination and quantification of pharmacological, physiological, or behavioral manipulations on ensembles of simultaneously recorded neurons in functionally related neural circuits. Journal of Neuroscience Methods 121(2): 181-198, 15 December, 2002

Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues. Biomaterials 27(36): 6083-6095, 2006

Mussel-inspired 3D fiber scaffolds for heart-on-a-chip toxicity studies of engineered nanomaterials. Analytical and Bioanalytical Chemistry 2018, 2018

Single fiber recording in the mammalian heart: Its role in the study of physiological and pharmacological mechanisms. Proc Western Pharmacol Soc 2: 34-46, 1959

Engineered cardiac tissues. Current Opinion in Biotechnology 22(5): 706-714, 2012

Microfluidic heart on a chip for higher throughput pharmacological studies. Lab on a Chip 13(18): 3599-3608, 2014

Strategies for improving the physiological relevance of human engineered tissues. Trends in Biotechnology 33(7): 401-407, 2016

Engineered heart tissue enables study of residual undifferentiated embryonic stem cell activity in a cardiac environment. Biotechnology and Bioengineering 108(3): 704-719, 2011

Pharmacological cardiac arrest in open heart surgery (experimental study). Archivio di Chirurgia del Torace 16: 191-204, 1959

The heart on a chip: the role of realistic mathematical models of cardiac electrical activity in understanding and treating cardiac arrhythmias. Heart Rhythm 4(6): 779-780, 2007

A tissue engineered contractile cardiac graft improves the cardiac performance in infarct rat heart. Circulation 104(17 Supplement): II 599, October 23, 2001

Congestive heart failure and sudden cardiac death: Pharmacological and non pharmacological treatment strategies. Zeitschrift für Kardiologie 89(Suppl 7): 55-59, 2000