Section 54
Chapter 53,003

Enzyme stabilization via cross-linked enzyme aggregates

Gupta, M.N.; Raghava, S.

Methods in Molecular Biology 679: 133-145


ISSN/ISBN: 1940-6029
PMID: 20865393
DOI: 10.1007/978-1-60761-895-9_11
Accession: 053002075

Download citation:  

Extensive cross-linking of a precipitate of a protein by a cross-linking reagent (glutaraldehyde has been most commonly used) creates an insoluble enzyme preparation called cross-linked enzyme aggregates (CLEAs). CLEAs show high stability and performance in both conventional aqueous media as well as nonaqueous media. These are also stable at fairly high temperatures. CLEAs having more than one kind of enzyme activity can be prepared and such CLEAs are called combi-CLEAs or multipurpose CLEAs. Extent of cross-linking often influences their morphology, stability, activity, and enantioselectivity.

PDF emailed within 0-6 h: $19.90