+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Epidemic of infectious laryngotracheitis in Italy: characterization of virus isolates by PCR-restriction fragment length polymorphism and sequence analysis



Epidemic of infectious laryngotracheitis in Italy: characterization of virus isolates by PCR-restriction fragment length polymorphism and sequence analysis



Avian Diseases 54(4): 1172-1177



Between May 2007 and October 2008, 34 outbreaks of mild to moderate forms of infectious laryngotracheitis (ILT) occurred in commercial broiler flocks in Italy. Affected birds showed watery eyes, conjunctivitis, nasal discharge, reduction of feed and water consumption, and gasping with expectoration of blood-stained mucus. The mortality rate was < 10%. Gross lesions consisted of conjunctivitis, excess of mucus, blood, or presence of diphtheritic membranes in trachea. A real-time PCR assay was performed to confirm the presence of ILT virus (ILTV) DNA in tracheal tissue homogenates. Twenty-three ILTV isolates were propagated on the chorion-allantoic membrane of embryonated chicken eggs showing typical plaques. PCR combined with restriction fragment length polymorphism and gene sequencing of isolates showed a high genetic correlation between field strains and chicken embryo origin vaccines.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053004876

Download citation: RISBibTeXText

PMID: 21313836

DOI: 10.1637/9398-051910-reg.1


Related references

Epidemic of Infectious Laryngotracheitis in Italy: Characterization of Virus Isolates by Pcr-Restriction Fragment Length Polymorphism and Sequence Analysis. Avian Diseases 54(4): 1172-1177, 2010

Characterization of western European field isolates and vaccine strains of avian infectious laryngotracheitis virus by restriction fragment length polymorphism and sequence analysis. Avian Diseases 52(2): 278-283, 2008

Characterization of field strains of infectious laryngotracheitis virus in China by restriction fragment length polymorphism and sequence analysis. Journal of Veterinary Diagnostic Investigation 28(1): 46-49, 2016

Characterization by restriction fragment length polymorphism and sequence analysis of field and vaccine strains of infectious laryngotracheitis virus involved in severe outbreaks. Avian Pathology 39(6): 425-433, 2010

Characterization of infectious laryngotracheitis virus isolates from commercial poultry by polymerase chain reaction and restriction fragment length polymorphism. Avian Diseases: 1, 59-63, 2008

Characterization of infectious laryngotracheitis virus isolates from the US by polymerase chain reaction and restriction fragment length polymorphism of multiple genome regions. Avian Pathology 36(2): 167-176, 2007

Characterization of infectious laryngotracheitis virus (ILTV) isolates from commercial poultry by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Avian Diseases 52(1): 59-63, 2008

Characterization of Infectious Laryngotracheitis Virus (Iltv) Isolates from Commercial Poultry by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (Pcr-Rflp). Avian Diseases 52(1): 59-63, 2008

Restriction fragment length polymorphism analysis of multiple genome regions of Korean isolates of infectious laryngotracheitis virus collected from chickens. Poultry Science 92(8): 2053-2058, 2013

Rapid differentiation of vaccine strains and field isolates of infectious laryngotracheitis virus by restriction fragment length polymorphism of PCR products. Journal of Virological Methods 66(2): 179-186, 1997

Analysis of Korean strains of infectious laryngotracheitis virus by nucleotide sequences and restriction fragment length polymorphism. Veterinary microbiology 83(4): 321-331, 2001

Rapid detection and characterization from field cases of infectious laryngotracheitis virus by real-time polymerase chain reaction and restriction fragment length polymorphism. Avian Pathology 35(2): 173-179, 2006

Differentiation of Infectious Laryngotracheitis Virus Isolates by Restriction Fragment Length Polymorphic Analysis of Polymerase Chain Reaction Products Amplified from Multiple Genes. Avian Diseases 50(1): 28-34, 2006

Differentiation of infectious laryngotracheitis virus isolates by restriction fragment length polymorphic analysis of polymerase chain reaction products amplified from multiple genes. Avian Diseases 50(1): 28-34, 2006

Genotypic characterization of Indian isolates of infectious bursal disease virus strains by reverse transcription-polymerase chain reaction combined with restriction fragment length polymorphism analysis. Acta Virologica 60(1): 34-40, 2016