+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Establishment of a transgenic hairy root system in wild and domesticated watermelon (Citrullus lanatus) for studying root vigor under drought



Establishment of a transgenic hairy root system in wild and domesticated watermelon (Citrullus lanatus) for studying root vigor under drought



Plant Cell Reports 29(7): 771-778



Root vigor is an important trait for the growth of terrestrial plants, especially in water-deficit environments. Although deserts plants are known for their highly developed root architecture, the molecular mechanism responsible for this trait has not been determined. Here we established an efficient protocol for the genetic manipulation of two varieties of watermelon plants: a desert-grown wild watermelon that shows vigorous root growth under drought, and a domesticated cultivar showing retardation of root growth under drought stress. Agrobacterium rhizogenes-mediated transgenic hairy roots were efficiently induced and selected from the hypocotyls of these plants. Transgenic GUS expression was detected in the roots by RT-PCR and histochemical GUS staining. Moreover, a liquid culture system for evaluating their root growth was also established. Interestingly, growth of the hairy roots derived from domesticated variety of watermelon strongly inhibited under high osmotic condition, whereas the hairy roots derived from wild variety of watermelon retained substantial growth rates under the stress condition. The new protocol presented here offers a powerful tool for the comparative study of the molecular mechanism underlying drought-induced root growth in desert plants.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053034697

Download citation: RISBibTeXText

PMID: 20445980

DOI: 10.1007/s00299-010-0863-3


Related references

Resistance to Southern Root-knot Nematode (Meloidogyne incognita) in Wild Watermelon (Citrullus lanatus var. citroides). Journal of Nematology 48(1): 14-19, 2016

Agrobacterium-mediated transformation system for the drought and excess light stress-tolerant wild watermelon (Citrullus lanatus). Plant Biotechnology 22(1): 13-18, 2005

Partial Leaf Chemical Profiles of a Desert Watermelon Species (Citrullus colocynthis) and Heirloom Watermelon Cultivars (Citrullus lanatus var. lanatus). Hortscience 47(5): 580-584, 2012

Resistance of watermelon (Citrullus lanatus var. citroides) germplasm to root-knot nematodes. Journal of Nematology 38(2): 298-298, 2006

Charcaterization of watermelon (Citrullus lanatus var. citroides) germplasm for resistance to root-knot nematodes. Hortscience 42(7): 1530-1533, 2007

A genetic linkage map for watermelon derived from a testcross population: (Citrullus lanatus var. citroides x C. lanatus var. lanatus) x Citrullus colocynthis. Theoretical and Applied Genetics 105(4): 555-563, 2002

A genetic linkage map for watermelon derived from a testcross population: ( Citrullus lanatus var. citroides x C. lanatus var. lanatus) x Citrullus colocynthis. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 105(4): 555-563, 2003

Effect of fungal fusaric acid on the root and leaf physiology of watermelon (Citrullus lanatus) seedlings. Plant and Soil 308(1-2): 255-266, 2008

Accessions of Citrullus lanatus var. citroides Are Valuable Rootstocks for Grafted Watermelon in Fields Infested with Root-knot Nematodes. Hortscience 50(1): 4-8, 2015

Effect of Nitrate/Ammonium Ratios on Growth, Root Morphology and NUTRIENT ELEMENTS UPTAKE OF WATERMELON (CITRULLUS LANATUS) SEEDLINGS. Journal of Plant Nutrition 37(11): 1859-1872, 2014

Influence of Citrullus lanatus var. citroides Rootstocks and Their F1 Hybrids on Yield and Response to Root-knot Nematode, Meloidogyne incognita, in Grafted Watermelon. Hortscience 50(1): 9-12, 2015

Impacts of a gravel-sand mulch and supplemental drip irrigation on watermelon (Citrullus lanatus Mats. & Nakai) root distribution and yield. Soil and Tillage Research 89(1): 35-44, 2006

Copper Based Nanomaterials Suppress Root Fungal Disease in Watermelon (Citrullus lanatus): Role of Particle Morphology, Composition and Dissolution Behavior. Acs Sustainable Chemistry and Engineering 6(11): 14847-14856, 2018

Optimization of ISSR-PCR system for cultivar verification in watermelon (Citrullus lanatus var. lanatus). Seed Science and Technology 39(2): 293-302, 2011

Resistance of watermelon (Citrullus lanatus Var. citroides) germplasm for resistance to root-knot nematodes. Hortscience 41(3): 520-520, 2006