+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Estrogen modulates iodoacetate-induced gene expression in bovine cartilage explants



Estrogen modulates iodoacetate-induced gene expression in bovine cartilage explants



Journal of Orthopaedic Research 28(5): 607-615



Estrogen loss may be involved in onset or progression of osteoarthritis. Estrogen receptors are present in chondrocytes, thus estrogen may exert effects directly on cartilage. However, studies on direct estrogen effects on cartilage are limited. We investigated, in an in vitro cartilage explant model, whether estrogen prevents damage or stimulates repair after damage induced by addition of iodoacetate (IA), as an experimental model for osteoarthritis. We used healthy bovine cartilage explants. Prevention experiment: Explants precultured with/without estradiol (E) for 3 days were cultured with IA for 4 h on day 0, and subsequently cultured as in preculture: with/without E. Explants were harvested at day 2 for gene expression analysis. Repair experiment: At day 0, explants were cultured with IA for 4 h on day 0, and subsequently cultured without E or with E. Explants were harvested at days 2, 10, and 14 for gene expression analysis. IA transiently downregulated most genes tested, whereas vascular endothelial growth factor (VEGF) was upregulated on day 2. On day 14, transforming growth factor beta (TGFB)1 and TGFB3 were upregulated, and matrix metalloproteinase (MMP)13 and VEGF downregulated. Estradiol affected gene expression of aggrecan (AGC)1, MMP2, MMP14, tissue inhibitor of metalloproteinase (TIMP)2, TGFB2, and TGFB3. Prevention experiment: Estradiol did not significantly affect IA-induced changes in gene expression (no significant interaction). Repair experiment: Estradiol affected IA-induced changes in expression of collagen (COL)2, MMP2, MMP3, MMP13, MMP14, TIMP2, TGFB2, TGFB3, and VEGF. Estradiol affects expression of anabolic and catabolic genes in bovine cartilage explants and modulates the effects of IA. These effects of estradiol may be beneficial for cartilage maintenance and repair.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053043054

Download citation: RISBibTeXText

PMID: 19953602

DOI: 10.1002/jor.21042


Related references

Mechanical Loading of Cartilage Explants with Compression and Sliding Motion Modulates Gene Expression of Lubricin and Catabolic Enzymes. Cartilage 6(3): 185-193, 2015

Gene expression profile of mechanically impacted bovine articular cartilage explants. Journal of Orthopaedic Research 23(5): 1146-1151, 2005

Cyclic compression-induced p38 activation and subsequent MMP13 expression requires Rho/ROCK activity in bovine cartilage explants. Inflammation Research 61(10): 1093-1100, 2012

Dithiocarbamates inhibit IL-1-induced cartilage degradation in bovine articular cartilage explants. Inflammation Research. 45(5): 215-217, 1996

Autologous solution protects bovine cartilage explants from IL-1α- and TNFα-induced cartilage degradation. Journal of Orthopaedic Research 31(12): 1929-1935, 2013

Effect of glucosamine and chondroitin sulfate on regulation of gene expression of proteolytic enzymes and their inhibitors in interleukin-1-challenged bovine articular cartilage explants. American Journal of Veterinary Research 66(11): 1870-1876, 2005

Glucosamine Hydrochloride but Not Chondroitin Sulfate Prevents Cartilage Degradation and Inflammation Induced by Interleukin-1α in Bovine Cartilage Explants. Cartilage 7(1): 70-81, 2016

Oleocanthal Modulates Estradiol-Induced Gene Expression Involving Estrogen Receptor α. Planta Medica 81(14): 1263-1269, 2016

A bovine hyaline cartilage proteinase inhibitor is active against some serine proteinases and inhibits the IL-1 alpha-mediated matrix degradation of bovine articular cartilage explants. Matrix . Supplement 1: 321-322, 1992

Stromelysin expression in IL-1 beta stimulated bovine articular cartilage explants. Inflammation Research 44(Suppl. 2): S119-S120, 1995

Effects of deer bone extract on the expression of pro-inflammatory cytokine and cartilage-related genes in monosodium iodoacetate-induced osteoarthritic rats. Bioscience Biotechnology and Biochemistry 78(10): 1703-1709, 2014

Effects of interleukin 1(IL1)-induced matrix breakdown on chondrocyte morphology in bovine nasal cartilage explants. Turk Veterinerlik ve Hayvanclk Dergisi 29(4): 951-957, 2005

Estrogen modulates oxytocin gene expression in regions of the rat supraoptic and paraventricular nuclei that contain estrogen receptor-beta. Progress in Brain Research 139: 15-29, 2002

Interleukin-4, an inhibitor of cartilage breakdown in bovine articular cartilage explants. Journal of Rheumatology 22(9): 1740-1746, 1995