+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Ethanol-responsive genes (Crtam, Zbtb16, and Mobp) located in the alcohol-QTL region of chromosome 9 are associated with alcohol preference in mice



Ethanol-responsive genes (Crtam, Zbtb16, and Mobp) located in the alcohol-QTL region of chromosome 9 are associated with alcohol preference in mice



Alcoholism, Clinical and Experimental Research 33(8): 1409-1416



Previously, our group identified cytotoxic and regulatory T-cell molecule (Crtam), zinc finger and BTB domain containing 16 (Zbtb16), and myelin-associated oligodendrocytic basic protein (Mobp) as ethanol-responsive genes in the mouse brain by gene expression profiling. In this study, we used a genetic co-segregation analysis to assess the association of Crtam, Zbtb16, and Mobp with the alcohol preference (AP) phenotype in the alcohol-preferring C57BL/6J (B6) and alcohol avoiding DBA/2J (D2) strains of mice. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to confirm previous microarray analysis results that Crtam, Zbtb16, and Mobp brain mRNA levels in the B6 and D2 strains are altered by ethanol treatment. The association of the 3 genes with AP was assessed in a F(2) population (n = 427) derived from the reciprocal crosses involving the B6 and D2 strains. Each F(2) individual was assessed for their AP using the 2 bottle choice test and genotyped for Crtam, Zbtb16, and Mobp single nucleotide polymorphisms (SNPs) that differ between B6 and D2 mice. Semi-quantitative RT-PCR analysis confirmed that Crtam, Zbtb16, and Mobp are ethanol-responsive genes. The SNP analyses show that alleles of the 3 genes co-segregate with the AP phenotype in F(2) mice, where individuals homozygous for the B6 allele have higher AP than those homozygous for the D2 allele. Also, the Crtam-Zbtb16 loci that are tightly linked and the Mobp locus act in an additive fashion in determining the relative AP phenotype. Our results are consistent with the hypothesis that Crtam, Zbtb16, and Mobp may be involved in AP in mice. The nature of this association remains to be established and may reflect a direct effect of these genes or an indirect effect caused by linked genes on mouse chromosome 9.

(PDF emailed within 0-6 h: $19.90)

Accession: 053045329

Download citation: RISBibTeXText

PMID: 19413645

DOI: 10.1111/j.1530-0277.2009.00971.x


Related references

Ethanol locomotor sensitization, but not tolerance correlates with selection for alcohol preference in high- and low-alcohol preferring mice. Psychopharmacology 151(2-3): 252-260, August, 2000

Different blood acetaldehyde concentration following ethanol administration in a newly developed high alcohol preference and low alcohol preference rat model system. Alcohol and Alcoholism 37(1): 9-12, 2002

Low alcohol preference among the high alcohol preference C57 strain of mice; preference increased by saline injections. Psychopharmacology 147(2): 182-189, 1999

EEG and ERP profiles in the high alcohol preferring and low alcohol preferring mice Relationship to ethanol preference. Brain Research 961(2): 243-254, 31 January, 2003

EEG and ERP profiles in the high alcohol preferring (HAP) and low alcohol preferring (LAP) mice: Relationship to ethanol preference. Brain Research 961(2): 243-254, 2003

Regulation of ethanol consumption alcohol preference in c 57bl 6j and dba 2j mice evidence for an oro sensory component in the avoidance of ethanol by dba 2j mice. Behavior Genetics 7(1): 43-44, 1977

Low alcohol preference among the "high alcohol preference" C57/BL10 mice; factors affecting such preference. Pharmacology, Biochemistry, and Behavior 72(1-2): 475-481, 2002

MRNA differential display identifies novel strain specific ethanol responsive cDNAs involved in alcohol preference. American Journal of Human Genetics 65(4): A273, 1999

The effects of 6R-L-erythro-5,6,7,8-tetrahydrobiopterin on ethanol-induced sleep time and ethanol elimination in inbred strains of mice with different alcohol preference. Neuropsychobiology 36(4): 188-193, 1997

Alcohol preference in ethanol preferring P rats is associated with altered HPA axis and neurosteroid responses to alcohol. Alcoholism Clinical & Experimental Research 24(5 Supplement): 49A, 2000

Effects of various serotonergic agents on alcohol intake and alcohol preference in Wistar rats selected at two different levels of alcohol preference. Alcohol and Alcoholism 28(2): 157-170, 1993

Genomewide SNP screen to detect quantitative trait loci for alcohol preference in the high alcohol preferring and low alcohol preferring mice. Alcoholism, Clinical and Experimental Research 33(3): 531-537, 2009

Repeated deprivations shift preference for higher ethanol concentrations and enhance the magnitude and duration of the alcohol deprivation effect in the alcohol preferring rat. Alcoholism Clinical & Experimental Research 24(5 Supplement): 14A, 2000