EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Ethylene insensitivity conferred by a mutated Arabidopsis ethylene receptor gene alters nodulation in transgenic Lotus japonicus


Annals of Botany 104(2): 277-285
Ethylene insensitivity conferred by a mutated Arabidopsis ethylene receptor gene alters nodulation in transgenic Lotus japonicus
Transgenics are used to demonstrate a causal relationship between ethylene insensitivity of a seedling legume plant, the level of ethylene receptor gene expression, lateral root growth and Mesorhizobium loti-induced nodule initiation. Lotus japonicus plants expressing the dominant etr1-1 allele of the Arabidopsis thaliana gene encoding a well-characterized mutated ethylene receptor were created by stable Agrobacterium tumefaciens transformation. Single insertion, homozygous lines were characterized for symbiotic properties. Transgenic plants were ethylene insensitive as judged by the lack of the 'Triple Response', and their continued ability to grow and nodulate in the presence of inhibitory concentrations of ACC (1-aminocyclopropane-1-carboxylic acid; an ethylene precursor). Transgenic plants with high insensitivity to ACC had significantly fewer lateral roots and exhibited increased nodulation while showing no altered nitrate sensitivity or lack of systemic autoregulation. Whereas ACC-insensitive shoot growth and nodulation were observed in transformants, root growth was inhibited similarly to the wild type. Increased nodulation was caused by increased infection and a seven-fold increase in nodules developing between xylem poles. Bacteroid numbers per symbiosome increased about 1.7-fold in ethylene-insensitive plants. The study further demonstrates multiple roles for ethylene in nodule initiation by influencing root cell infections and radial positioning, independent of autoregulation and nitrate inhibition of nodulation.


Accession: 053050569

PMID: 19505874

DOI: 10.1093/aob/mcp132



Related references

Transgenic Lotus japonicus with an ethylene receptor gene Cm-ERS1. Plant and cell physiology 45(4): 427-435, 2004

Ethylene insensitivity conferred by Arabidopsis ERS gene. Science 269(5231): 1712-1714, 1995

Effects of ethylene precursor and inhibitors for ethylene biosynthesis and perception of nodulation in Lotus japonicus and Macroptilium atropurpureum. Plant & Cell Physiology 41(7): 893-897, July, 2000

Effects of ethylene precursor and inhibitors for ethylene biosynthesis and perception on nodulation in Lotus japonicus and Macroptilium atropurpureum. Plant & Cell Physiology 41(7): 893-897, 2000

Transgenic Lotus japonicus with an Ethylene Receptor Gene Cm-ERSJ/H70A Enhances Formation of Infection Threads and Nodule Primordia. Plant and Cell Physiology 45(4): 7-35, 2004

Transgenic Lotus japonicus with an ethylene receptor gene Cm-ERS1/H70A enhances formation of infection threads and nodule primordia. Plant & Cell Physiology 45(4): 427-435, 2004

The relationship between ethylene binding and dominant insensitivity conferred by mutant forms of the ETR1 ethylene receptor. Plant Physiology 121(1): 291-300, 1999

Regulated ethylene insensitivity through the inducible expression of the Arabidopsis etr1-1 mutant ethylene receptor in tomato. Plant Physiology 152(4): 1928-1939, 2010

Insensitivity to Ethylene Conferred by a Dominant Mutation in Arabidopsis thaliana. Science 241(4869): 1086-1089, 1988

Recessive and dominant mutations in the ethylene biosynthetic gene ACS5 of Arabidopsis confer cytokinin insensitivity and ethylene overproduction, respectively. Proceedings of the National Academy of Sciences of the United States of America 95(8): 4766-4771, 1998