Section 54
Chapter 53,083

Evaluation of the Genetic Toxicity of Cyclopentane and Ammonium Nitrate - In vitro Mammalian Chromosomal Aberration Assay in Chinese Hamster Ovary Cells

Kim, S-Jin.; Rim, K-Taek.; Kim, J-Kyu.; Kim, H-Yeong.; Yang, J-Sun.

Safety and Health at Work 2(1): 17-25


ISSN/ISBN: 2093-7911
PMID: 22953183
DOI: 10.5491/shaw.2011.2.1.17
Accession: 053082411

Download citation:  

In this study, the in vitro mammalian chromosomal aberration (CA) assay was conducted to gain additional information concerning the hazards associated with the use of cyclopentane and ammonium nitrate. While these two chemicals had already been tested by many methods, they had not been studied in the CA test. The assay was performed using the ovarian infantile cell (CHO-K1 cell), by the direct method (-S9) and by the metabolic activated method (+S9 mix). Using the direct method, the 7 dosages in a 48 hour treatment group did not show that the frequency of CA is proportion to the dosage addition. The frequency of CA is not proportion to the dosage addition for a 6 hour treatment using the metabolic activated method. From these findings, it was decided that the 2 chemicals do not induce chromosomal aberrations under the tested conditions.

PDF emailed within 0-6 h: $19.90