+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Exploring the site-selective binding of jatrorrhizine to human serum albumin: spectroscopic and molecular modeling approaches



Exploring the site-selective binding of jatrorrhizine to human serum albumin: spectroscopic and molecular modeling approaches



Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy 117: 163-169



This paper exploring the site-selective binding of jatrorrhizine to human serum albumin (HSA) under physiological conditions (pH=7.4). The investigation was carried out using fluorescence spectroscopy, UV-vis spectroscopy, and molecular modeling. The results of fluorescence quenching and UV-vis absorption spectra experiments indicated the formation of the complex of HSA-jatrorrhizine. Binding parameters calculating from Stern-Volmer method and Scatchard method were calculated at 298, 304 and 310 K, with the corresponding thermodynamic parameters ΔG, ΔH and ΔS as well. Binding parameters calculating from Stern-Volmer method and Scatchard method showed that jatrorrhizine bind to HSA with the binding affinities of the order 10(4) L mol(-1). The thermodynamic parameters studies revealed that the binding was characterized by negative enthalpy and positive entropy changes and the electrostatic interactions play a major role for jatrorrhizine-HSA association. Site marker competitive displacement experiments and molecular modeling calculation demonstrating that jatrorrhizine is mainly located within the hydrophobic pocket of the subdomain IIIA of HSA. Furthermore, the synchronous fluorescence spectra suggested that the association between jatrorrhizine and HSA changed molecular conformation of HSA.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053142909

Download citation: RISBibTeXText

PMID: 23988532

DOI: 10.1016/j.saa.2013.08.013


Related references

Determining the binding site and binding affinity of estradiol to human serum albumin and holo-transferrin: fluorescence spectroscopic, isothermal titration calorimetry and molecular modeling approaches. Journal of Biomolecular Structure and Dynamics 36(7): 1747-1763, 2017

Spectroscopic analysis and molecular modeling on the interaction of jatrorrhizine with human serum albumin (HSA). Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy 118: 48-54, 2015

Binding site identification of metformin to human serum albumin and glycated human serum albumin by spectroscopic and molecular modeling techniques: a comparison study. Journal of Biomolecular Structure and Dynamics 33(3): 513-533, 2016

Exploring the binding mechanism of dihydropyrimidinones to human serum albumin: spectroscopic and molecular modeling techniques. Colloids and Surfaces. B, Biointerfaces 84(1): 272-279, 2011

Exploring binding properties of sertraline with human serum albumin: Combination of spectroscopic and molecular modeling studies. Chemico-Biological Interactions 242: 235-246, 2016

Interaction of Jatrorrhizine with Human Gamma Globulin in membrane mimetic environments: Probing of the binding mechanism and binding site by spectroscopic and molecular modeling methods. Journal of Molecular Structure 980(1-3): 108-113, 2010

Spectroscopic and molecular modeling approaches to investigate the binding of proton pump inhibitors to human serum albumin. Journal of Biomolecular Structure and Dynamics 35(15): 3205-3220, 2016

Biophysical studies on the interactions of jatrorrhizine with bovine serum albumin by spectroscopic and molecular modeling methods. Molecular Biology Reports 40(7): 4397-4404, 2014

Interaction of coomassie brilliant blue G250 with human serum albumin: Probing of the binding mechanism and binding site by spectroscopic and molecular modeling methods. Journal of Molecular Structure 968(1-3): 24-31, 2010

Probing the binding interaction of human serum albumin with three bioactive constituents of Eriobotrta japonica leaves: Spectroscopic and molecular modeling approaches. Journal of Photochemistry and Photobiology. B, Biology 148: 268-276, 2016

Investigating the site selective binding of busulfan to human serum albumin: Biophysical and molecular docking approaches. International Journal of Biological Macromolecules 107(Pt B): 1414-1421, 2017

Separate and simultaneous binding effects through a non-cooperative behavior between cyclophosphamide hydrochloride and fluoxymesterone upon interaction with human serum albumin: multi-spectroscopic and molecular modeling approaches. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy 88: 177-191, 2012

Comparing the interaction of cyclophosphamide monohydrate to human serum albumin as opposed to holo-transferrin by spectroscopic and molecular modeling methods: evidence for allocating the binding site. Protein and Peptide Letters 17(12): 1524-1535, 2011

Exploring the mechanism of interaction between sulindac and human serum albumin: Spectroscopic and molecular modeling methods. Journal of Luminescence 138: 8-14, 2013

Characterization of the myricetin-human serum albumin complex by spectroscopic and molecular modeling approaches. Biomacromolecules 8(7): 2182-2189, 2007