+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Expression analysis of genes associated with sucrose accumulation in sugarcane (Saccharum spp. hybrids) varieties differing in content and time of peak sucrose storage



Expression analysis of genes associated with sucrose accumulation in sugarcane (Saccharum spp. hybrids) varieties differing in content and time of peak sucrose storage



Plant Biology 17(3): 608-617



Sucrose synthesis/accumulation in sugarcane is a complex process involving many genes and regulatory sequences that control biochemical events in source-sink tissues. Among these, sucrose synthase (SuSy), sucrose phosphate synthase (SPS), soluble acid (SAI) and cell wall (CWI) invertases are important. Expression of these enzymes was compared in an early (CoJ64) and late (BO91) maturing sugarcane variety using end-point and qRT-PCR. Quantitative RT-PCR at four crop stages revealed high CWI expression in upper internodes of CoJ64, which declined significantly in both top and bottom internodes with maturity. In BO91, CWI expression was high in top and bottom internodes and declined significantly only in top internodes as the crop matured. Overall, CWI expression was higher in CoJ64 than in BO91. During crop growth, there was no significant change in SPS expression in bottom internodes in CoJ64, whereas in BO91 it decreased significantly. Apart from a significant decrease in expression of SuSy in mature bottom internodes of BO91, there was no significant change. Similar SAI expression was observed with both end-point and RT-PCR, except for significantly increased expression in top internodes of CoJ64 with maturity. SAI, being a major sucrose hydrolysing enzyme, was also monitored with end-point PCR expression in internode tissues of CoJ64 and BO91, with higher expression of SAI in BO91 at early crop stages. Enzyme inhibitors, e.g. manganese chloride (Mn(++) ), significantly suppressed expression of SAI in both early- and late-maturing varieties. Present findings enhance understanding of critical sucrose metabolic gene expression in sugarcane varieties differing in content and time of peak sucrose storage. Thus, through employing these genes, improvement of sugarcane sucrose content is possible.

(PDF emailed within 0-6 h: $19.90)

Accession: 053145968

Download citation: RISBibTeXText

PMID: 25311688

DOI: 10.1111/plb.12276


Related references

Differential expression of soluble acid invertase genes in the shoots of high-sucrose and low-sucrose species of Saccharum and their hybrids. Australian Journal of Plant Physiology 27(3): 193-199, 2000

Expression analysis of genes associated with sucrose accumulation and its effect on source-sink relationship in high sucrose accumulating early maturing sugarcane variety. Physiology and Molecular Biology of Plants 25(1): 207-220, 2019

Changes in endogenous hormone content and its relation to sucrose accumulation in sugarcane (Saccharum officinarum L.). Southwest China Journal of Agricultural Sciences 20(3): 392-394, 2007

Expression profiling of sucrose metabolizing genes in Saccharum, Sorghum and their hybrids. Applied Biochemistry and Biotechnology 174(4): 1510-1519, 2015

Effects of flowering on sucrose content and sucrose yield in five sugarcane varieties. Proceedings of the Annual Congress South African Sugar Technologists' Association ( 63): 181-185, 1989

Expression analysis of genes associated with sucrose accumulation in sugarcane under normal and GA(3)-induced source-sink perturbed conditions. Acta Physiologiae Plantarum 39(6): 133, 2017

Optimization of Callus Induction and Plant Regeneration in Sugarcane (Saccharum spp.) for a Study of Sucrose Accumulation in Relation to Soluble Acid Invertase Expression. Chiang Mai Journal of Science 42(4): 797-805, 2015

Functional analysis of sucrose phosphate synthase (SPS) and sucrose synthase (SS) in sugarcane (Saccharum) cultivars. Plant Biology 13(2): 325-332, 2011

Structure and expression of soluble acid invertase genes in the stem of high- and low-sucrose accumulating Saccharum species and hybrids. Sugar 2000 Symposium: Sugarcane: research towards efficient and sustainable production: 102-104, 1996

Study of total solids-sucrose content relationship in sugarcane juice with reference to varieties. VI. Reduction of sucrose yields in Co. 419 due to predominance of inferior total solids-sucrose relationship-patterns. Indian J. Sugarcane Res. Developm, 7: 242-47, 1963

The influence of time of harvest and age of sixteen sugarcane varieties on their sucrose content. J. Agric. Univ. PR. 44: 1-10, 1960

Influence of time of harvest and age of sixteen sugarcane varieties on their sucrose content. Jour Agric Univ Puerto Rico 44(1): 1-10, 1960

Correlation analysis between sucrose content and the activities of four enzymes related to sucrose metabolism in sugarcane internodes. 2007

Molecular biology of sucrose storage in sugarcane saccharum officinarum. Journal of Cellular Biochemistry Supplement (14 PART E): 351, 1990

Study of total solids -sucrose content relationship in sugarcane juice with reference to varieties. III. Determination of earliness or lateness of varieties using total solids - sucrose regression. Indian J. Sugarcane Res. Developm, 7: 118-26, 1963