+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Expression and functional role of β3 -adrenoceptors in the human ureter

Expression and functional role of β3 -adrenoceptors in the human ureter

International Journal of Urology 20(10): 1007-1014

To investigate the presence of β-adrenoceptor subtypes in the human ureter, and to examine whether β(3) -adrenoceptors modulate relaxation of the human ureter. Expression of messenger ribonucleic acid of β-adrenoceptors in the human ureter was determined by reverse transcription polymerase chain reaction, and distribution of β-adrenoceptors was examined by immunohistochemistry. In functional studies, the relaxant effects of isoproterenol, procaterol, TRK-380, salbutamol and BRL 37344 on KCl-induced contraction of the human ureter were evaluated, and the inhibitory effects of isoproterenol, procaterol and TRK-380 on electrical field stimulation-induced contractions were determined. Expression of β(1) -, β(2) - and β(3) -adrenoceptor messenger ribonucleic acid in the human ureter was confirmed by reverse transcription polymerase chain reaction. Positive staining for β(1) -, β(2) - and β(3) -adrenoceptor was identified not only in smooth muscle, but also in the urothelium of the human ureter. All β-adrenoceptor agonists decreased the tone of KCl-induced contractions of the human ureter with a rank order of relaxant effects of isoproterenol > procaterol > TRK-380 > salbutamol > BRL 37344. Furthermore, isoproterenol, procaterol and TRK-380 significantly decreased the amplitude of electrical field stimulation-induced contractions with a rank order of inhibitory effects of isoproterenol > procaterol > TRK-380. Human ureteral relaxation is mediated by both β(2) - and β(3) -adrenoceptor stimulation. β(3) -Adrenoceptor agonists have the potential to relax the human ureter, and their clinical application in the treatment of ureteral stones is expected.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 053147106

Download citation: RISBibTeXText

PMID: 23360304

DOI: 10.1111/iju.12093

Related references

Expression and functional role of ?3-adrenoceptors in the human ureter. 2013

Expression and functional role of beta-adrenoceptors in the human urinary bladder urothelium. Naunyn-Schmiedeberg's Archives of Pharmacology 377(4-6): 473-481, 2008

Cardiostimulant and cardiodepressant effects through overexpressed human beta2-adrenoceptors in murine heart: regional differences and functional role of beta1-adrenoceptors. Naunyn-Schmiedeberg's Archives of Pharmacology 367(4): 380-390, 2003

Existence Of A 3-Adrenoceptor And Its Functional Role In The Human Ureter. The Journal of Urology 164(4): 1364-1370, 2000

Functional role of beta3-adrenoceptors in neurogenic human detrusors. Neurourology & Urodynamics 18(4): 352-353, 1999

Functional role of beta2-adrenoceptors in the transplanted human heart. Journal of Cardiovascular Pharmacology 30(6): 811-816, 1997

Existence of a beta3-adrenoceptro and its functional role in the human ureter. Journal of Urology 164(4): 1364-1370, 2000

Expression and functional coupling of liver β2 - adrenoceptors in the human hepatocellular carcinoma. Pharmacology 89(5-6): 313-320, 2012

The functional role of typical and atypical beta adrenoceptors in rat and human adipocytes. European Journal of Pharmacology 183(3): 1001, 1990

The phorbol ester Tpa stimulates the expression of functional -adrenoceptors in human T lymphoblasts Molt 3. Biochemical Journal 261(3): 959-963, 1989

Atypical beta-adrenoceptors mediating relaxation in the human colon: functional evidence for beta3-rather than beta4-adrenoceptors. Pharmacological Research 39(5): 345-348, 1999

The phorbol ester TPA stimulates the expression of functional beta-adrenoceptors in human T lymphoblasts Molt 3. Biochemical Journal 261(3): 959-963, 1989

Expression of functional postjunctional alpha 2-adrenoceptors in rabbit isolated distal saphenous artery--a permissive role for angiotensin II?. British Journal of Pharmacology 96(2): 259-261, 1989

Characterization of cyclic nucleotide phosphodiesterase isoenzymes in the human ureter and their functional role in vitro. World Journal Of Urology. 12(5): 286-291, 1994

Expression level and role in ureteral contraction of alpha1-adrenoceptor subtypes in human ureter. The Journal of Urology 179(4): 559, 2008