+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Expression, purification, crystallization and preliminary crystallographic analysis of hepatitis B virus core protein dimerized via a peptide linker containing an EGFP insertion



Expression, purification, crystallization and preliminary crystallographic analysis of hepatitis B virus core protein dimerized via a peptide linker containing an EGFP insertion



Acta Crystallographica. Section F Structural Biology and Crystallization Communications 69(Pt 8): 942-945



Virus-like particles (VLPs) have many potentially useful applications. The core proteins of human hepatitis B virus self-assemble into icosahedral VLPs. As previously reported, core protein dimers (CPDs), produced by connecting two core proteins via a peptide linker, can also assemble into VLPs. CPDs in which heterologous proteins were connected to the C-terminus (CPD1) were found to rearrange into symmetrical octahedra during crystallization. In this study, a heterologous protein was inserted into the peptide linker of the CPD (CPD2). CPD2 was expressed in Escherichia coli, assembled into VLPs, purified and crystallized. A single crystal diffracted to 2.8 Å resolution and belonged to the cubic space group F432, with unit-cell parameters a = b = c = 218.6 Å. Single-crystal analysis showed that CPD1 and CPD2 rearranged into the same octahedral organization in a crystallization solution.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053160024

Download citation: RISBibTeXText

PMID: 23908049

DOI: 10.1107/s1744309113019957


Related references

Expression, purification, crystallization and preliminary X-ray crystallographic studies of hepatitis B virus core fusion protein corresponding to octahedral particles. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 69(Pt 2): 165-169, 2013

Crystallization and preliminary crystallographic analysis of the fusion core of the spike protein of the murine coronavirus mouse hepatitis virus (MHV). Acta Crystallographica. Section D, Biological Crystallography 60(Pt 11): 2013-2015, 2004

Cloning, expression, purification, crystallization and preliminary crystallographic study of the protein module (BIV2-Helix) in the fusion core of bovine immunodeficiency-like virus (BIV) gp40. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 61(Pt 2): 249-251, 2005

Crystallization and preliminary X-ray crystallographic analysis of the trimer core from measles virus fusion protein. Acta Crystallographica Section D Biological Crystallography 59(3): 587-590, 2003

Purification, crystallization and preliminary X-ray crystallographic analysis of the nucleocapsid protein of Bunyamwera virus. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications 62(Pt 4): 361-364, 2006

Expression, purification, crystallization and preliminary crystallographic analysis of the proliferation-associated protein Ebp1. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 63(Pt 9): 768-770, 2007

Expression, purification, crystallization and preliminary crystallographic analysis of osmotically inducible protein C. Acta Crystallographica. Section D Biological Crystallography 60(Pt 2): 357-358, 2004

Crystallization and preliminary X-ray crystallographic analysis of the helicase domain of hepatitis C virus NS3 protein. Acta Crystallographica Section D Biological Crystallography. 54(1): 121-123,. 1, 1998

Expression, purification, crystallization and preliminary X-ray crystallographic study of the nucleocapsid protein of tomato spotted wilt virus. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 69(Pt 6): 700-703, 2013

Expression, purification, crystallization and preliminary crystallographic analysis of human myotubularin-related protein 3. Acta Crystallographica. Section F Structural Biology Communications 70(Pt 9): 1240-1243, 2014

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the histone-like HU protein from Spiroplasma melliferum KC3. Acta Crystallographica. Section F Structural Biology Communications 71(Pt 1): 24-27, 2015

Purification, crystallization and preliminary X-ray crystallographic analysis of the human heat-shock protein 40 Hdj1 and its C-terminal peptide-binding domain. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 66(Pt 12): 1591-1595, 2010

Expression, crystallization and preliminary crystallographic study of mouse hepatitis virus (MHV) nucleocapsid protein C-terminal domain. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 66(Pt 6): 674-676, 2010