+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Flavobacterium johnsoniae PorV is required for secretion of a subset of proteins targeted to the type IX secretion system

Flavobacterium johnsoniae PorV is required for secretion of a subset of proteins targeted to the type IX secretion system

Journal of Bacteriology 197(1): 147-158

Flavobacterium johnsoniae exhibits gliding motility and digests many polysaccharides, including chitin. A novel protein secretion system, the type IX secretion system (T9SS), is required for gliding and chitin utilization. The T9SS secretes the cell surface motility adhesins SprB and RemA and the chitinase ChiA. Proteins involved in secretion by the T9SS include GldK, GldL, GldM, GldN, SprA, SprE, and SprT. Porphyromonas gingivalis has orthologs for each of these that are required for secretion of gingipain protease virulence factors by its T9SS. P. gingivalis porU and porV have also been linked to T9SS-mediated secretion, and F. johnsoniae has orthologs of these. Mutations in F. johnsoniae porU and porV were constructed to determine if they function in secretion. Cells of a porV deletion mutant were deficient in chitin utilization and failed to secrete ChiA. They were also deficient in secretion of the motility adhesin RemA but retained the ability to secrete SprB. SprB is involved in gliding motility and is needed for formation of spreading colonies on agar, and the porV mutant exhibited gliding motility and formed spreading colonies. However, the porV mutant was partially deficient in attachment to glass, apparently because of the absence of RemA and other adhesins on the cell surface. The porV mutant also appeared to be deficient in secretion of numerous other proteins that have carboxy-terminal domains associated with targeting to the T9SS. PorU was not required for secretion of ChiA, RemA, or SprB, indicating that it does not play an essential role in the F. johnsoniae T9SS.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 053258748

Download citation: RISBibTeXText

PMID: 25331433

DOI: 10.1128/jb.02085-14

Related references

Flavobacterium johnsoniae chitinase ChiA is required for chitin utilization and is secreted by the type IX secretion system. Journal of Bacteriology 196(5): 961-970, 2014

Flavobacterium johnsoniae GldK, GldL, GldM, and SprA are required for secretion of the cell surface gliding motility adhesins SprB and RemA. Journal of Bacteriology 195(14): 3201-3212, 2013

SpiC is required for secretion of Salmonella Pathogenicity Island 2 type III secretion system proteins. Cellular Microbiology 4(8): 531-540, 2002

The Type IX Secretion System Is Required for Virulence of the Fish Pathogen Flavobacterium columnare. Applied and Environmental Microbiology 83(23):, 2017

Diverse C-Terminal Sequences Involved in Flavobacterium johnsoniae Protein Secretion. Journal of Bacteriology 199(12):, 2017

Mutations in Flavobacterium johnsoniae sprE result in defects in gliding motility and protein secretion. Journal of Bacteriology 193(19): 5322-5327, 2011

Subset of hybrid eukaryotic proteins is exported by the type I secretion system of Erwinia chrysanthemi. Journal of Bacteriology 183(4): 1346-1358, 2001

Riemerella anatipestifer Type IX Secretion System Is Required for Virulence and Gelatinase Secretion. Frontiers in Microbiology 8: 2553, 2017

An A/U-Rich Enhancer Region Is Required for High-Level Protein Secretion through the HlyA Type I Secretion System. Applied and Environmental Microbiology 84(1):, 2018

Flavobacterium compostarboris sp. nov., isolated from leaf-and-branch compost, and emended descriptions of Flavobacterium hercynium, Flavobacterium resistens and Flavobacterium johnsoniae. International Journal of Systematic and Evolutionary Microbiology 62(Pt 8): 2018-2024, 2012

An optimal set of features for predicting type IV secretion system effector proteins for a subset of species based on a multi-level feature selection approach. Plos one 13(5): E0197041, 2018

The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan. Microbiology 146: 2237-2248, 2000

Type 3 Secretion System Island Encoded Proteins Required for Colonization by Non-O1/non-O139 Serogroup Vibrio cholerae. Infection and Immunity 83(7): 2862-2869, 2015

Flavobacterium gliding motility and the type IX secretion system. Current Opinion in Microbiology 28: 72-77, 2015

Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. Journal of Biological Chemistry 286(14): 12317-12327, 2011