+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Fluorescence Imaging with One-Nanometer Accuracy (FIONA) of Cy3-DNA under Deoxygenation Conditions



Fluorescence Imaging with One-Nanometer Accuracy (FIONA) of Cy3-DNA under Deoxygenation Conditions



Csh Protocols 2007: Pdb.Prot4868



INTRODUCTIONFIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. This protocol provides the steps necessary to run a control experiment, using DNA labeled with Cy3, to assess the efficacy of the FIONA setup and the level of attainable resolution. The Cy3-DNA is immobilized on a coverslip and imaged under deoxygenation conditions. It is important that the fluorophores remain photostable throughout the experiment. This requires an oxygen-scavenging system (e.g., glucose oxidase and catalase) in the medium.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 053265530

Download citation: RISBibTeXText

PMID: 21356956


Related references

Fluorescence imaging with one-nanometer accuracy (FIONA). Journal of Visualized Experiments 2014(91): 51774, 2014

Equipment Setup for Fluorescence Imaging with One-Nanometer Accuracy (FIONA). Csh Protocols 2007: Pdb.Ip45, 2007

Data Analysis Methods for Fluorescence Imaging with One-Nanometer Accuracy (FIONA). Csh Protocols 2007: Pdb.Ip46, 2007

Constructing Sample Chambers for Fluorescence Imaging with One-Nanometer Accuracy (FIONA). Csh Protocols 2007: Pdb.Prot4867, 2007

Fluorescence imaging with one nanometer accuracy: application to molecular motors. Accounts of Chemical Research 38(7): 574-582, 2005

Fluorescence imaging with one nanometer accuracy: in vitro and in vivo studies of molecular motors. Methods in Molecular Biology 778: 33-56, 2011

High accuracy FIONA-AFM hybrid imaging. Ultramicroscopy 111(5): 350-355, 2011

Fluorescence axial localization with nanometer accuracy and precision. Nano Letters 12(7): 3731-3735, 2012

Ultra-stable and versatile widefield cryo-fluorescence microscope for single-molecule localization with sub-nanometer accuracy. Optics Express 23(3): 3770-3783, 2015

Axial nanometer distances measured by fluorescence lifetime imaging microscopy. Nano Letters 10(4): 1497-1500, 2010

Imaging nanometer-sized α-synuclein aggregates by superresolution fluorescence localization microscopy. Biophysical Journal 102(7): 1598-1607, 2012

Fluorescence interference and energy transfer imaging of nanometer-scale topography at an intermembrane junction. Biophysical Journal 82(1 Part 2): 160a, 2002

Correlative cathodoluminescence and near-infrared fluorescence imaging for bridging from nanometer to millimeter scale bioimaging. Microscopy 63(Suppl. 1): I29, 2014

5-Aminolevulinic acid-derived tumor fluorescence: the diagnostic accuracy of visible fluorescence qualities as corroborated by spectrometry and histology and postoperative imaging. Neurosurgery 74(3): 310, 2014

Seesawed fluorescence nano-aptasensor based on highly vertical ZnO nanorods and three-dimensional quantitative fluorescence imaging for enhanced detection accuracy of ATP. Biosensors and Bioelectronics 90: 450-458, 2017