+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Follicle development in cryopreserved bitch ovarian tissue grafted to immunodeficient mouse



Follicle development in cryopreserved bitch ovarian tissue grafted to immunodeficient mouse



Reproduction Fertility and Development 24(3): 461-471



The present study evaluated: (1) in vivo follicular development in canine ovarian tissue after slow freezing and xenotransplantation; and (2) the use of erythropoietin (EPO) as an angiogenic factor to optimise the transplantation procedure. Frozen-thawed ovarian tissue from five bitches was grafted into severe combined immunodeficient (SCID) mice (n=47) treated with or without EPO (500 IU kg(-1), once daily for 3 days) (Groups A and B, respectively) and analysed after 0, 1, 8 or 16 weeks. Follicle grade, follicle density, follicle morphology and stromal cells density were assessed by histological analysis, whereas vascularisation of the graft was quantified by immunohistochemistry with anti-α-smooth muscle actin antibody. Despite a massive loss of follicles after grafting, secondary follicle density was higher at 8 and 16 weeks than at 1 week regardless of EPO treatment. EPO significantly improved early follicle morphology and stromal cell density after 8 weeks and blood vessel density at 16 weeks after transplantation (P<0.05). Intact secondary follicles with more than three granulosa cells layers were observed 16 weeks after transplantation. The results suggest that canine ovarian tissue can be successfully preserved by our slow-freezing protocol because the tissue showed follicular growth after xenotransplantation. EPO treatment did not lessen the massive loss of follicles after transplantation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053274633

Download citation: RISBibTeXText

PMID: 22401278

DOI: 10.1071/rd11166


Related references

Variation in follicle health and development in cultured cryopreserved ovarian cortical tissue: a study of ovarian tissue from patients undergoing fertility preservation. Human Fertility 2019: 1-11, 2019

Development of antral follicles in cryopreserved cat ovarian tissue transplanted to immunodeficient mice. Theriogenology 15 61(2-3): 581-594, 2004

Development of antral follicles in cryopreserved cat ovarian tissue transplanted to immunodeficient mice. Theriogenology 61(2-3): 581-594, 2004

Follicle Development of Xenotransplanted Sheep Ovarian Tissue into Male and Female Immunodeficient Rats. International Journal of Fertility and Sterility 9(3): 354-360, 2015

Antral follicle development in xenograft of cryopreserved elephant ovarian tissue. Cryobiology 35(4): 356-357, 1997

Histological changes following HCG administration in human cryopreserved ovarian tissue transplanted in immunodeficient mice. Human Reproduction 16(Abstract Book 1): 21, 2001

In vitro follicular development of cryopreserved mouse ovarian tissue. Reproduction 130(2): 187-192, 2005

A simple method to quantify follicle survival in cryopreserved human ovarian tissue. Human Reproduction 33(12): 2276-2284, 2018

Significant loss of primordial follicles after prolonged gonadotropin stimulation in xenografts of cryopreserved human ovarian tissue in severe combined immunodeficient mice. Fertility and Sterility 87(1): 195-197, 2007

Oocyte maturation, follicle rupture and luteinization in human cryopreserved ovarian tissue following xenografting. Human Reproduction 18(9): 1772-1781, 2003

Transplantation of cryopreserved human ovarian tissue results in follicle growth initiation in SCID mice. Fertility & Sterility 73(3): 599-603, 2000

Human single follicle growth in vitro from cryopreserved ovarian tissue after slow freezing or vitrification. Human Reproduction 31(4): 763-773, 2016

Effect of different cryoprotectant agents on spermatogenesis efficiency in cryopreserved and grafted neonatal mouse testicular tissue. Cryobiology 67(1): 70-75, 2013

Supplementation of culture media with vitamin E improves mouse antral follicle maturation and embryo development from vitrified ovarian tissue. Journal of Obstetrics and Gynaecology Research 42(5): 526-535, 2016

Advanced follicle development in xenografted prepubertal ovarian tissue: the common marmoset as a nonhuman primate model for ovarian tissue transplantation. Fertility and Sterility 95(4): 1428-1434, 2011