+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Functional validation of Capsicum frutescens aminotransferase gene involved in vanillylamine biosynthesis using Agrobacterium mediated genetic transformation studies in Nicotiana tabacum and Capsicum frutescens calli cultures



Functional validation of Capsicum frutescens aminotransferase gene involved in vanillylamine biosynthesis using Agrobacterium mediated genetic transformation studies in Nicotiana tabacum and Capsicum frutescens calli cultures



Plant Science 195: 96-105



Capsaicinoid biosynthesis involves the participation of two substrates viz. vanillylamine and C(9)-C(11) fatty acid moieties. Vanillylamine which is a derivative of vanillin is synthesized through a transaminase reaction in the phenylpropanoid pathway of capsaicinoid synthesis. Here we report the functional validation of earlier reported putative aminotransferase gene for vanillylamine biosynthesis in heterologous system using Agrobacterium mediated genetic transformation studies in Nicotiana tabacum and Capsicum frutescens calli cultures. Molecular analysis tools comprising PCR and Southern blot analysis have shown the integration of the foreign gene in N. tabacum and C. frutescens calli cultures. The study shows the production of vanillylamine in transformed N. tabacum callus cultures and also the reduction of vanillylamine production when whole gene based antisense binary vector construct was used in transformation of C. frutescens callus cultures. Vanillylamine production, aminotransferase assay with Western blot analysis for crude proteins of transformants established the production of putative aminotransferase (pAMT) protein in alternate plant. The result is a clear evidence of involvement of the reported putative aminotransferase responsible for vanillylamine biosynthesis in capsaicinoid biosynthesis pathway, confirming the gene function through functional validation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053330058

Download citation: RISBibTeXText

PMID: 22921003

DOI: 10.1016/j.plantsci.2012.06.014


Related references

Biotransformation of ferulic acid and vanillylamine to capsaicin and vanillin in immobilized cell cultures of Capsicum frutescens. Plant cell tissue and organ culture 44(2): 117-121, 1996

Final report on the safety assessment of capsicum annuum extract, capsicum annuum fruit extract, capsicum annuum resin, capsicum annuum fruit powder, capsicum frutescens fruit, capsicum frutescens fruit extract, capsicum frutescens resin, and capsaicin. International Journal of Toxicology 26(Suppl. 1): 3-106, 2007

Mathematical Model For Prediction Of Moisture Content In JalapeO Pepper ( Capsicum frutescens ) Modelo MatemTico Para La PredicciN Del Contenido De Humedad En Chile JalapeO ( Capsicum frutescens ) Modelo MatemTico Para A PredicciN Do Contido De Humidade En Chile JalapeO ( Capsicum frutescens ). Ciencia y Tecnologia Alimentaria 4(3): 154-157, 2004

Studies on the regulation of capsaicin biosynthesis in immobilized cell cultures and the developing fruit of the chilli pepper capsicum frutescens. Morris, P , Et Al (Ed ) Secondary Metabolism in Plant Cell Cultures; International Association Of Plant Cell And Tissue Culture Meeting, Sheffield, England, July 1985 Xi+262p Cambridge University Press: New York, New York, Usa; Cambridge, England Illus 121-127, 1986

Capsaicin biosynthesis in cell cultures of Capsicum frutescens. Plant and animal cells: process possibilities editors C Webb and F Mavituna: 3, 1987

Nutrient interactions and compositional nutrient diagnosis norms in a semi-domesticated cultivar of Capsicum frutescens FT Interacciones nutrimentales Y normas de diagnostico de nutrimento compuesto en UN cultivar semidomesticado de Capsicum frutescens. 2007

The capsaicin alkaloid content of capsicum d fruits at different stages of maturity capsicum minimum d capsicum frutescens d. Lloydia 31(3): 272-274, 1968

Cytogentical relationships among three species of chili peppers, Capsicum chinense, Capsicum frutescens and Capsicum baccatum. Ikushugaku zasshi = Japanese journal of breeding 34(1): 50-56, 1984

Cytogenetic relationships among 3 spp. of chili peppers capsicum chinense capsicum frutescens and capsicum baccatum. Japanese Journal of Breeding 34(1): 50-56, 1984

A low-pungency S3212 genotype of Capsicum frutescens caused by a mutation in the putative aminotransferase (p-AMT) gene. Molecular Genetics and Genomics 290(6): 2217-2224, 2015

Variations in infectivity among populations of meloidogyne javanica on tobacco nicotiana tabacum cultivar nc 95 and pepper capsicum frutescens cultivar california wonder. Plant Disease 65(1): 65-66, 1981

Studies on virus disease of chillies capsicum annuum and capsicum frutescens in rajasthan. Indian Journal of Mycology and Plant Pathology 5(1): 29, 1975

Cytogenetics studies on the interspecific hybrid capsicum baccatum l. times capsicum frutescens l. and its progeny. Euphytica 59(2-3): 135-140, 1992

Cytogenetic studies on the f 1 hybrid capsicum chinense x capsicum frutescens l. Genetica Agraria 41(1): 47-56, 1987

De novo transcriptome assembly in chili pepper (Capsicum frutescens) to identify genes involved in the biosynthesis of capsaicinoids. Plos one 8(1): E48156, 2013