EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Fungal ecdysteroid-22-oxidase, a new tool for manipulating ecdysteroid signaling and insect development


Fungal ecdysteroid-22-oxidase, a new tool for manipulating ecdysteroid signaling and insect development



Journal of Biological Chemistry 287(20): 16488-16498



ISSN/ISBN: 0021-9258

PMID: 22427652

DOI: 10.1074/jbc.m112.341180

Steroid hormones ecdysteroids regulate varieties of developmental processes in insects. Although the ecdysteroid titer can be increased experimentally with ease, its artificial reduction, although desirable, is very difficult to achieve. Here we characterized the ecdysteroid-inactivating enzyme ecdysteroid-22-oxidase (E22O) from the entomopathogenic fungus Nomuraea rileyi and used it to develop methods for reducing ecdysteroid titer and thereby controlling insect development. K(m) and K(cat) values of the purified E22O for oxidizing ecdysone were 4.4 μM and 8.4/s, respectively, indicating that E22O can inactivate ecdysone more efficiently than other ecdysteroid inactivating enzymes characterized so far. The cloned E22O cDNA encoded a FAD-dependent oxidoreductase. Injection of recombinant E22O into the silkworm Bombyx mori interfered with larval molting and metamorphosis. In the hemolymph of E22O-injected pupae, the titer of hormonally active 20-hydroxyecdysone decreased and concomitantly large amounts of inactive 22-dehydroecdysteroids accumulated. E22O injection also prevented molting of various other insects. In the larvae of the crambid moth Haritalodes basipunctalis, E22O injection induced a diapause-like developmental arrest, which, as in normal diapause, was broken by chilling. Transient expression of the E22O gene by in vivo lipofection effectively decreased the 20-hydroxyecdysone titer and blocked molting in B. mori. Transgenic expression of E22O in Drosophila melanogaster caused embryonic morphological defects, phenotypes of which were very similar to those of the ecdysteroid synthesis deficient mutants. Thus, as the first available simple but versatile tool for reducing the internal ecdysteroid titer, E22O could find use in controlling a broad range of ecdysteroid-associated developmental and physiological phenomena.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053332849

Download citation: RISBibTeXText

Related references

The effect of manipulating ecdysteroid signaling on embryonic eye development in the locust Schistocerca americana. Development Genes and Evolution 213(12): 587-600, 2003

Ecdysteroid signaling in ecdysteroid-resistant cell lines from the polyphagous noctuid pest Spodoptera exigua. Insect Biochemistry and Molecular Biology 38(9): 825-833, 2008

Ecdysteroid titer during metamorphosis and the effect of ecdysteroid on oocyte development in Phormia regina. Korean Journal of Applied Entomology 31(4): 371-378, 1992

Activity of an anti-ecdysteroid compound (KK-42) on ovarian development and ecdysteroid secretion in mealworms. Mededelingen Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen, Universiteit Gent 62(2b): 531-537, 1997

The effects of ingested ecdysteroid agonists and an ecdysteroid antagonist on larval development of two polyphagous lepidopterans and. Entomologia Experimentalis et Applicata 83(3): 263-276, 1997

An unsuspected ecdysteroid/steroid phosphatase activity in the key T-cell regulator, Sts-1: surprising relationship to insect ecdysteroid phosphate phosphatase. Proteins 67(3): 720-731, 2007

Development of a cell-based assay for ecdysteroid quantification using an early ecdysteroid-inducible gene promoter. Applied Entomology and Zoology 49(3): 443-452, 2014

Development of an assay for ecdysteroid like and anti ecdysteroid activities in plants. Hrdy, I (Ed ) Insect Chemical Ecology; Conference, Tabor, Czechoslovakia, August 12-18, 1990 Xiii+513p Spb Academic Publishing Bv: The Hague, Netherlands; Academia Publishing House Of The Czechoslovak Academy Of Sciences: Prague, Czechoslovakia Illus 221-226, 1991

Ecdysteroid release and ecdysteroid titer during larval-adult development of the Mediterranean field cricket, Gryllus bimaculatus. European Journal of Entomology 92(1): 81-92, 1995

Effects of ovariectomy and allatectomy on ecdysteroid synthesis and ecdysteroid titers during larval-adult development of Gryllus bimaculatus de Geer (Ensifera: Gryllidae). Archives of Insect Biochemistry and Physiology 35(1/2): 149-158, 1997

Hemolymph ecdysteroid titer and ecdysteroid-dependent developmental events in the last-larval stadium of the silkworm, Bombyx mori: role of low ecdysteroid titer in larval-pupal metamorphosis and a reappraisal of the head critical period. Journal of Insect Physiology 44(10): 867-881, 1998

Regulation of ecdysteroid signaling: cloning and characterization of ecdysone oxidase: a novel steroid oxidase from the cotton leafworm, Spodoptera littoralis. Journal of Biological Chemistry 276(29): 26819-26828, 2001

The effects of ingested ecdysteroid agonists (20-hydroxyecdysone, RH5849 and RH5992) and an ecdysteroid antagonist (cucurbitacin B) on larval development of two polyphagous lepidopterans (Acherontia atropos and Lacanobia oleracea). Entomologia Experimentalis et Applicata 83(3): 263-276, 1997

26-hydroxylation of ecdysteroids is catalyzed by a typical cytochrome P-450-dependent oxidase and related to ecdysteroid resistance in an insect cell line. European Journal of Biochemistry 248(3): 707-716, 1997

Ecdysteroid 7,9(11)-dien-6-ones as potential photoaffinity labels for ecdysteroid binding proteins. Journal of Insect Science Tucson 2(11): 1-11, 2002