Section 54
Chapter 53,388

Genetic transformation mediated by piggy Bac in the Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae)

Liu, D.; Yan, S.; Huang, Y.; Tan, A.; Stanley, D.W.; Song, Q.

Archives of Insect Biochemistry and Physiology 80(3): 140-150


ISSN/ISBN: 1520-6327
PMID: 22696097
DOI: 10.1002/arch.21035
Accession: 053387598

The Asian corn borer, Ostrinia furnacalis, is a serious pest of corn, sorghum, and cotton in China and other Asian countries. The present study is the first attempt to establish the transgenic line in O. furnacalis using a piggyBac transposon, which will shed light on the future genetic control of O. furnacalis. A piggyBac vector pBac[A3EGFP] was constructed to express enhanced green fluorescence protein (EGFP)under the control of Bombyx mori actin3 promoter. Transient EGFP expression was detected 48 h after preblastodermic microinjection of pBac[A3EGFP] and the excision assay showed the transgenic vector was precisely excised. In G1 animals, PCR (polymerase chain reaction)-based investigations revealed that the exogenous gene had been introduced into O. furnacalis genome and expressed at the transcriptional level. Western blot analysis showed EGFP expression at the protein level, indicating the heritability of the transgene.

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