+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Genetic variability within the Polish tomato torrado virus Kra isolate caused by deletions in the 3'-untranslated region of genomic RNA1



Genetic variability within the Polish tomato torrado virus Kra isolate caused by deletions in the 3'-untranslated region of genomic RNA1



Virus Research 185: 47-52



Tomato torrado virus (ToTV) is in the genus Torradovirus in the family Secoviridae. ToTV contains a single-stranded, positive-sense, bipartite RNA genome encapsidated in icosahedral particles. It is a serious tomato pathogen causing significant crop reductions. Its occurrence has been reported from many countries worldwide. However, the state of knowledge of ToTV epidemiology, sequences and phylogeny is still rather poor. In this study we found that the Polish ToTV isolates are characterized by significant genetic variability of the 3'-untranslated region (UTR) of RNA1. The high resolution melting real-time PCR approach showed the presence of genetic variants within Polish ToTV isolates purified from Nicotiana benthamiana. Further sequencing of Kra ToTV revealed five genetic variants of RNA1 within the isolate differing in the 3'-untranslated region length resulting from deletions ranging from 6 to 163 nucleotides. In light of the published studies, the genetic variability of ToTV associated with large deletions within an isolate may not necessarily be rare and may influence the virus evolution and adaptation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053387971

Download citation: RISBibTeXText

PMID: 24657785

DOI: 10.1016/j.virusres.2014.03.008


Related references

The nucleotide sequence of a Polish isolate of Tomato torrado virus. Virus Genes 37(3): 400-406, 2008

Characterization of the partial RNA1 and RNA2 3 untranslated region of Tomato ringspot virus isolates from North America. Canadian Journal of Plant Pathology 33(1): 94-99, 2011

Epidemics of Tomato torrado virus, Pepino mosaic virus and Tomato chlorosis virus in tomato crops: do mixed infections contribute to torrado disease epidemiology?. Annals of Applied Biology 156(3): 401-410, 2010

The N-terminal fragment of the tomato torrado virus RNA1-encoded polyprotein induces a hypersensitive response (HR)-like reaction in Nicotiana benthamiana. Archives of Virology 161(7): 1849-1858, 2016

Evolution of the Bunyamwera virus polymerase to accommodate deletions within genomic untranslated region sequences. Journal of Virology 89(7): 3957-3964, 2015

Biological and molecular characterization of Polish isolates of Tomato torrado virus. Journal Of Phytopathology8: 1, 56-62, 2010

The role of genetic variability in the 5 untranslated region ofRAD51in Polish newborns with HCMV infection. Molecular Genetics, Microbiology and Virology 29(4): 204-210, 2014

Complete sequence of the RNA1 of a European isolate of tomato chlorosis virus. Archives of Virology 152(4): 839-841, 2006

Genome Characteristics, Phylogeny and Varying Host Specificity of Polish Kra and Ros Isolates of Tomato torrado virus. Journal of Phytopathology 164(4): 281-285, 2016

RNA1-Independent Replication and GFP Expression from Tomato marchitez virus Isolate M Cloned cDNA. Phytopathology 106(5): 500-509, 2016

Cap-independent translational enhancement by the 3' untranslated region of red clover necrotic mosaic virus RNA1. Journal of Virology 77(22): 12113-12121, 2003

The genomic RNA1 and RNA2 sequences of the tobacco rattle virus isolates found in Polish potato fields. Virus Research 185: 110-113, 2014

Cloning and sequencing of full-length cDNAs of RNA1 and RNA2 of a Tomato black ring virus isolate from Poland. Archives of Virology 149(4): 799-807, 2004

The 3'-untranslated region of RNA1 as a primary determinant of temperature sensitivity of Red clover necrotic mosaic virus Canadian strain. Virology 293(2): 320-327, 2002

Restriction analysis of genetic variability of Polish isolates of Tomato black ring virus. Acta Biochimica Polonica 51(3): 673-681, 2004