+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Genomic RNA editing and its impact on Ebola virus adaptation during serial passages in cell culture and infection of guinea pigs

Genomic RNA editing and its impact on Ebola virus adaptation during serial passages in cell culture and infection of guinea pigs

Journal of Infectious Diseases 204(Suppl. 3): S941

Synthesis of the structural, surface glycoprotein (GP) of Ebola virus (EBOV) is dependent on transcriptional RNA editing phenomenon. Editing results in the insertion of an extra adenosine by viral polymerase at the editing site (7 consecutive template uridines) during transcription of GP gene of the wild-type virus (EBOV/7U). In this study, we demonstrate that passage of EBOV/7U in Vero E6 cells results in the appearance and rapid accumulation of a variant (EBOV/8U) containing an additional uridine at the editing site in the viral genome. EBOV/8U outgrows and eventually replaces the wild-type EBOV during 4-5 passages. On the contrary, infection of guinea pigs with EBOV/8U leads to the appearance and rapid predominance by EBOV/7U. These rapid conversions suggest that editing of the genomic RNA occurs at a higher frequency than previously thought. In addition, it indicates that the EBOV/7U phenotype has a selective advantage that is linked to controlled expression of GP and/or expression of secreted sGP, the primary gene product for wild-type EBOV. This study demonstrates the potential for insertion and deletion of uridines in the editing site of the EBOV genomic RNA, depending on environmental constraints.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 053398161

Download citation: RISBibTeXText

PMID: 21987773

DOI: 10.1093/infdis/jir321

Related references

Transmission of swine fever to guinea pigs by serial passages; rapid loss of the virus virulence in pigs since the first passage of the virus in guinea pigs. 1978

Hematological and immunological parameters during Ebola virus passages in guinea-pigs. Voprosy Virusologii 51(4): 32-37, 2006

Ebola GP-specific monoclonal antibodies protect mice and guinea pigs from lethal Ebola virus infection. Plos Neglected Tropical Diseases 6(3): E1575, 2012

Efficacy of Ebola Glycoprotein-Specific Equine Polyclonal Antibody Product Against Lethal Ebola Virus Infection in Guinea Pigs. Journal of Infectious Diseases 218(Suppl_5): S603, 2018

Genomic changes detected after serial passages in cell culture of virulent human G1P[8] rotaviruses. Infection Genetics and Evolution 45: 6-10, 2016

Attempts to establish an immunoprophylaxis for transmissible gastroenteritis virus infection of pigs. i. pathogenicity of the B1 strain after serial passages. Zentralblatt fur Veterinarmedizin: Reihe B: 24 (9) 753-763, 1977

Pathogenesis of experimental Ebola virus infection in guinea pigs. Journal of Infectious Diseases 179(Suppl. 1): S203, 1999

Genotyping of varicella-zoster virus strains after serial passages in cell culture. Journal of Virological Methods 145(1): 80-83, 2007

A Single Amino Acid Change in the Marburg Virus Glycoprotein Arises during Serial Cell Culture Passages and Attenuates the Virus in a Macaque Model of Disease. Msphere 3(1):, 2018

Ebola virus infection in guinea pigs: Presumable role of granulomatous inflammation in pathogenesis. Archives of Virology 141(5): 909-921, 1996

Kunjin virus replicon-based vaccines expressing Ebola virus glycoprotein GP protect the guinea pig against lethal Ebola virus infection. Journal of Infectious Diseases 204(Suppl. 3): S1060-5, 2011

Impact of systemic or mucosal immunity to adenovirus on Ad-based Ebola virus vaccine efficacy in guinea pigs. Journal of Infectious Diseases 204(Suppl. 3): S1032, 2011

Mutational events in consecutive passages of hepatitis A virus strain GBM during cell culture adaptation. Virology 204(1): 60-68, 1994

Incomplete growth of simian virus 40 in African green monkey kidney cell culture induced by serial undiluted passages. Japanese Journal of Medical Science and Biology 18(5): 268, 1965

Effect of the host cell on the generation of defective Simian Virus 40 during undiluted serial passages and persistent infection. Virology 108(2): 525-530, 1981