EurekaMag
+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Glycosylation site-specific analysis of clade C HIV-1 envelope proteins


Glycosylation site-specific analysis of clade C HIV-1 envelope proteins



Journal of Proteome Research 8(9): 4231-4242



ISSN/ISBN: 1535-3893

PMID: 19610667

DOI: 10.1021/pr9002728

The extensive glycosylation of HIV-1 envelope proteins (Envs), gp120/gp41, is known to play an important role in evasion of host immune response by masking key neutralization epitopes and presenting the Env glycosylation as "self" to the host immune system. The Env glycosylation is mostly conserved but continues to evolve to modulate viral infectivity. Thus, profiling Env glycosylation and distinguishing interclade and intraclade glycosylation variations are necessary components in unraveling the effects of glycosylation on Env's immunogenicity. Here, we describe a mass spectrometry-based approach to characterize the glycosylation profiles of two rVV-expressed clade C Envs by identifying the glycan motifs on each glycosylation site and determining the degree of glycosylation site occupancy. One Env is a wild-type Env, while the other is a synthetic "consensus" Env (C.CON). The observed differences in the glycosylation profiles between the two clade C Envs show that C.CON has more unutilized sites and high levels of high mannose glycans; these features mimic the glycosylation profile of a Group M consensus immunogen, CON-S. Our results also reveal a clade-specific glycosylation pattern. Discerning interclade and intraclade glycosylation variations could provide valuable information in understanding the molecular differences among the different HIV-1 clades and in designing new Env-based immunogens.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053430076

Download citation: RISBibTeXText

Related references

Glycosylation site-specific analysis of HIV envelope proteins (JR-FL and CON-S) reveals major differences in glycosylation site occupancy, glycoform profiles, and antigenic epitopes' accessibility. Journal of Proteome Research 7(4): 1660-1674, 2008

Global site-specific N-glycosylation analysis of HIV envelope glycoprotein. Nature Communications 8: 14954, 2017

Role of clade B and clade A envelope glycosylation in HIV-1 tropism, transmission and neutralization susceptibility. Journal of Medical Primatology 32(4-5): 281, 2003

Site-Specific N-glycosylation Analysis of Recombinant Proteins by LC/MSE. Methods in Molecular Biology 2271: 133-154, 2021

Site-specific O-Glycosylation Analysis of Human Blood Plasma Proteins. Molecular and Cellular Proteomics: Mcp 15(2): 624-641, 2016

Carbohydrates on Proteins: Site-Specific Glycosylation Analysis by Mass Spectrometry. Annual Review of Analytical Chemistry 8: 463-483, 2015

Analysis of glycosylation site occupancy reveals a role for Ost3p and Ost6p in site-specific N-glycosylation efficiency. Molecular and Cellular Proteomics: Mcp 8(2): 357-364, 2009

Site-specific glycosylation of human recombinant erythropoietin: analysis of glycopeptides or peptides at each glycosylation site by fast atom bombardment mass spectrometry. Biochemistry 27(23): 8618-8626, 1988

Glycosylation and disulfide bond analysis of transiently and stably expressed clade C HIV-1 gp140 trimers in 293T cells identifies disulfide heterogeneity present in both proteins and differences in O-linked glycosylation. Journal of Proteome Research 13(9): 4012-4027, 2014

Synthetic glycosylation of proteins using N -( -saccharide) iodoacetamides: applications in site-specific glycosylation and solid-phase enzymic oligosaccharide synthesis. Biochemical Journal 300(3): 843-850, 1994

Cross-clade envelope glycoprotein 160-specific CD8+ cytotoxic T lymphocyte responses in early HIV type 1 clade B infection. Aids Research and Human Retroviruses 14(11): 925-937, 1998

Site-specific N-glycosylation analysis of animal cell culture-derived Zika virus proteins. Scientific Reports 11(1): 5147, 2021

Synthetic glycosylation of proteins using N-(beta-saccharide) iodoacetamides: applications in site-specific glycosylation and solid-phase enzymic oligosaccharide synthesis. Biochemical Journal 300: 843-850, 1994

Site-specific analysis of changes in the glycosylation of proteins in liver cirrhosis using data-independent workflow with soft fragmentation. Analytical and Bioanalytical Chemistry 409(2): 619-627, 2017

The Patterns of Coevolution in Clade B HIV Envelope's N-Glycosylation Sites. Plos one 10(6): E0128664, 2015