+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Gonadal transcriptomic analysis of yellow catfish (Pelteobagrus fulvidraco): identification of sex-related genes and genetic markers

Gonadal transcriptomic analysis of yellow catfish (Pelteobagrus fulvidraco): identification of sex-related genes and genetic markers

Physiological Genomics 46(21): 798-807

Yellow catfish (Pelteobagrus fulvidraco) has been recognized as a vital freshwater aquaculture species in East and Southeast Asia. In addition to its commercial interest, it is also attracted much attention because of its value in studying sex-determination mechanisms. A comprehensive gonadal transcriptome analysis is believed to provide a resource for genome annotation, candidate gene identification, and molecular marker development. Herein, we performed a de novo assembly of yellow catfish gonad transcriptome by high-throughput Illumina sequencing. A total of 82,123 contigs were obtained, ranging from 351 to 21,268 bp, and N50 of 2,329 bp. Unigenes of 21,869 in total were identified. Of these, 229 and 1,188 genes were found to be specifically expressed in XY gonad tissue for 1 yr and 2 yr old yellow catfish, respectively; correspondingly, 51 and 40 genes were identified in XX gonad tissue at those two stages. Gene ontology and KEGG analysis were conducted and classified all contigs into different categories. A large number of unigenes involved in sex determination were identified, as well as microsatellites and SNP variants. The expression patterns of sex-related genes were then validated by quantitative real-time PCR (qRT-PCR) suggesting the high reliability of RNA-Seq results. In this study, the transcriptome of yellow catfish gonad was first sequenced, assembled, and characterized; it provides a valuable genomic resource for better understanding of yellow catfish sex determination as well as development of molecular markers, thereby assisting in the production of monosex yellow catfish for aquaculture.

(PDF emailed within 0-6 h: $19.90)

Accession: 053433729

Download citation: RISBibTeXText

PMID: 25185028

DOI: 10.1152/physiolgenomics.00088.2014

Related references

Transcriptomic Analyses Reveal Novel Genes with Sexually Dimorphic Expression in Yellow Catfish (Pelteobagrus fulvidraco) Brain. Marine Biotechnology 17(5): 613-623, 2016

RAPD markers between yellow catfish Pelteobagrus fulvidraco and long yellow catfish Pelteobagrus eupogon. 2006

Characterization of immune-related genes in the yellow catfish Pelteobagrus fulvidraco in response to LPS challenge. Fish and Shellfish Immunology 56: 248-254, 2017

RAPD (random amplification of polymorphic DNA) markers between yellow catfish (Pelteobagrus fulvidraco) and long whiskers yellow catfish (P. eupogon). 2006

Serum biochemistry, histology and transcriptomic profile analysis reflect liver inflammation and damage following dietary histamine supplementation in yellow catfish (Pelteobagrus fulvidraco). Fish and Shellfish Immunology 77: 83-90, 2018

Potential contributions of heat shock proteins and related genes in sexual differentiation in yellow catfish (Pelteobagrus fulvidraco). Fish Physiology and Biochemistry 43(2): 465-475, 2017

Identification of differentially expressed genes in the spleens of polyriboinosinic polyribocytidylic acid (poly I:C)-stimulated yellow catfish Pelteobagrus fulvidraco. Fish and Shellfish Immunology 56: 278-285, 2017

Isolation and characterization of 57 novel polynucleotide microsatellites from yellow catfish Pelteobagrus fulvidraco genome for genetic analysis. Conservation Genetics Resources 6(1): 73-77, 2014

Identification of five genes in endoplasmic reticulum (ER) stress-apoptosis pathways in yellow catfish Pelteobagrus fulvidraco and their transcriptional responses to dietary lipid levels. Fish Physiology and Biochemistry 2019, 2019

Molecular identification and expression analysis of a goose-type lysozyme (LysG) gene in yellow catfish Pelteobagrus fulvidraco. Fish and Shellfish Immunology 58: 423-428, 2016

Three unsaturated fatty acid biosynthesis-related genes in yellow catfish Pelteobagrus fulvidraco: Molecular characterization, tissue expression and transcriptional regulation by leptin. Gene 563(1): 1-9, 2015

Effects of 17α-Methyltestosterone and Aromatase Inhibitor Letrozole on Sex Reversal, Gonadal Structure, and Growth in Yellow Catfish Pelteobagrus fulvidraco. Biological Bulletin 228(2): 108-117, 2015

Parentage determination of yellow catfish (Pelteobagrus Fulvidraco) based on microsatellite DNA markers. Aquaculture International 24(2): 567-576, 2016

Isolation and characterization of polymorphic microsatellite markers in the yellow catfish, Pelteobagrus fulvidraco. Conservation Genetics Resources 1(1): 63-66, 2009

Population structure and genetic diversity in yellow catfish ( Pelteobagrus fulvidraco ) assessed with microsatellites. Journal of Genetics 98(1), 2019