+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Greatly extended storage stability of electrochemical DNA biosensors using ternary thiolated self-assembled monolayers

Greatly extended storage stability of electrochemical DNA biosensors using ternary thiolated self-assembled monolayers

Talanta 99: 155-160

While high storage stability of sequence-selective DNA biosensors is crucial towards their routine applications, commonly used electrochemical hybridization biosensors are characterized with limited storage stability. In this article we demonstrate that recently developed ternary thiolated monolayers impart dramatic improvement in the storage stability of DNA electrochemical biosensors. In particular, highly stable multicomponent interfaces are prepared by co-immobilizing the thiolated capture probe (SHCP) with 1,6-hexanedithiol (HDT) on gold substrates, followed by the incorporation of 6-mercapto-1-hexanol (MCH) diluent. The resulting (SHCP/HDT+MCH) DNA hybridization recognition platform offers substantially higher storage stability compared to conventional binary (SHCP+MCH) monolayers. The (SHCP/HDT+MCH) ternary monolayers maintain their initial signal (S)-to-noise (N) ratio (S/N) over a prolonged 3 months period upon storage at 4 °C, compared to the rapid sensitivity loss observed using the common binary interfaces. This attractive stability performance promises the convenient usage of pre-prepared electrodes after prolonged time storage without any treatment. Such dramatic improvements in the storage stability have been achieved through a rational optimization of the concentration ratio of the SHCP and the other components of the ternary SAM. The improved storage stability of SHCP/HDT+MCH interfaces observed at higher concentrations of SHCP is attributed to a hindered displacement of SHCP by MCH in the resulting compact layers. The ability to design highly stable nucleic acid interfaces using common chemicals obviates the need of using specialized expensive reagents.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 053443848

Download citation: RISBibTeXText

PMID: 22967535

DOI: 10.1016/j.talanta.2012.05.033

Related references

The rise of self-assembled monolayers for fabricating electrochemical biosensors--an interfacial perspective. Chemical Record 12(1): 92, 2012

Electrochemical stability of self-assembled monolayers on nanoporous Au. Physical Chemistry Chemical Physics 13(26): 12277-12284, 2011

Chemical, electrochemical, and structural stability of low-density self-assembled monolayers. Langmuir 23(20): 10184-9, 2007

Electrochemical stability of self-assembled alkylphosphate monolayers on conducting metal oxides. Langmuir 27(16): 9890-9894, 2011

Comparison of the signaling and stability of electrochemical DNA sensors fabricated from 6- or 11-carbon self-assembled monolayers. Langmuir 22(25): 10796-10800, 2006

Improving the stability and sensing of electrochemical biosensors by employing trithiol-anchoring groups in a six-carbon self-assembled monolayer. Analytical Chemistry 81(3): 1095-1100, 2009

Electrochemical investigations on stability and protonation behavior of pyridine-terminated aromatic self-assembled monolayers. Physical Chemistry Chemical Physics 13(34): 15530-8, 2011

Design of Therapeutic Self-Assembled Monolayers of Thiolated Abiraterone. Nanomaterials 8(12):, 2018

Room-temperature ionic liquids as media to enhance the electrochemical stability of self-assembled monolayers of alkanethiols on gold electrodes. Chemical Communications 2005(3): 360-362, 2005

Development of amperometric biosensors using thiolated tetrathiafulvalene-derivatised self-assembled monolayer modified electrodes. Sensors and Actuators B: Chemical 134(2): 974-980, 2008

Bimodal atomic force microscopy for the characterization of thiolated self-assembled monolayers. Nanoscale 10(48): 23027-23036, 2018

Interfacial supramolecular self-assembled monolayers of C(60) by thiolated beta-cyclodextrin on gold surfaces via monoanionic C(60). Journal of the American Chemical Society 129(16): 4973-4980, 2007

Self-assembled monolayers for biosensors. Analyst 122(4): 43r-50r, 1997

Self-assembled monolayers in electroanalytical chemistry Application of omega-mercaptocarboxylic acid monolayers for electrochemical determination of ultralow levels of cadmium. Analytical Chemistry 66(1): 58-63, 1994

Adsorption dynamics and electrochemical and photophysical properties of thiolated ruthenium 2,2'-bipyridine monolayers. Journal of Physical Chemistry. B 110(20): 10063-9, 2006