+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Green-tissue-specific, C(4)-PEPC-promoter-driven expression of Cry1Ab makes transgenic potato plants resistant to tuber moth (Phthorimaea operculella, Zeller)



Green-tissue-specific, C(4)-PEPC-promoter-driven expression of Cry1Ab makes transgenic potato plants resistant to tuber moth (Phthorimaea operculella, Zeller)



Plant Cell Reports 28(12): 1869-1879



An important strategy for obtaining a safer transgenic plant may be the use of a spatial- or tissue-specific promoter, instead of a constitutive one. In this study, we have used a light-inducible maize PEPC promoter to regulate the cry1Ab gene, aiming to produce transgenic potatoes that are resistant to potato tuber moth (PTM) (Phthorimaea operculella, Zeller). Out of 60 regenerated lines having normal phenotypes, 55 lines were PCR-positive for both the cry1Ab and nptII genes. Southern analysis on three selected putative transgenic lines revealed that they have only a single intact copy of the cry1Ab gene. An investigation of the Cry1Ab protein in the leaves and light-exposed (LE) tubers of the transgenic lines demonstrated the presence of the protein in the foliage and green tubers but not in the light-not exposed (LNE) tubers. A bioassay analysis of excised leaves of nine randomly selected lines showed that eight lines had 100% PTM larval mortality. Confirming results were obtained in six selected lines using the whole plant bioassay in the greenhouse. LE transgenic tubers also exhibited 100% larval mortality; however, the levels of damage to the LNE transgenic tubers were high and statistically the same as those incurred by the non-transgenic ones. Based on the results, we believe that this spatial expression of Cry1Ab using the light-inducible PEPC promoter can control PTM infestation in the field and significantly reduce pollution transmission to storage potatoes.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053444923

Download citation: RISBibTeXText

PMID: 19882159

DOI: 10.1007/s00299-009-0790-3


Related references

Green-tissue-specific, C-4-PEPC-promoter-driven expression of Cry1Ab makes transgenic potato plants resistant to tuber moth (Phthorimaea operculella, Zeller) (vol 28, pg 1869, 2009). Plant Cell Reports 29(1): 111-111, 2010

Field evaluation of potato plants transgenic for a cry1Ac gene conferring resistance to potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae). Crop protection 25(3): 216-224, 2006

Morphometric and electrophoretic characterization of the potato tuber moth Phthorimaea operculella Zeller Caratterizzazione morfometrica ed elettroforetica di popolazioni di Phthorimaea operculella Zeller Lepidoptera Gelechiidae, tignola della patata. Atti del Congresso Nazionale Italiano di Entomologia 17: 163-164, 1994

Expression of various biotin-binding proteins in transgenic tobacco confers resistance to potato tuber moth, Phthorimaea operculella (Zeller) (fam. Gelechiidae). Transgenic Research 19(6): 1041-1051, 2010

Plants used in controlling the potato tuber moth, Phthorimaea operculella (Zeller). Crop Protection 14(8): 631-636, 1995

The breeding potential of wild potato species resistant to the potato tuber moth phthorimaea operculella zeller. Euphytica 39(2): 123-132, 1988

Promoter and piggyBac activities within embryos of the potato tuber moth, Phthorimaea operculella, Zeller (Lepidoptera: Gelechiidae). Gene 342(2): 293-301, 2004

Growth and development of the potato tuber moth, Phthorimaea operculella (Zeller), on resistant and susceptible potato genotypes in storage. Philippine Entomologist 9(1): 15-27, 1993

The reaction of host plants on the biology of potato tuber moth, Phthorimaea operculella (Zeller). Research on Crops 1(2): 196-200, 2000

Field and storage genetic resistance of the new transgenic potato variety cv. Costanera to the potato tuber moth Phthorimaea operculella (Zeller) in an arid-saline agroecosystem. IDESIA 20(2): 119-135, 2002

The influence of selected insecticides and insecticide-fertilizer combinations against the potato tuber moth, Phthorimaea operculella (Zeller), on potato plants. Bulletin of the Entomological Society of Egypt, Economic Series ( 20): 15-23, 1993

Susceptibility of potato plants grown from tubers irradiated with stimulation doses of gamma irradiation to potato tuber moth, Phthorimaea operculella Zeller (Lep., Gelechiidae). Journal of Applied Entomology 123(3): 159-164, 1999

Life cycle of the potato tuber moth Phthorimaea operculella Zeller Lepidoptera Gelechiidae in four commercial varieties of potato in Peru Ciclo biologico de la polilla de la papa Phthorimaea operculella Zeller Lepidoptera Gelechiidae en cuatro variedades comerciales de papa en Peru. Revista Peruana de Entomologia. 20 Octubre; 41: 75-78, 1999

Study of effect of temperatures on the natural death and the biotic potential of potato tuber moth, Phthorimaea operculella (Zeller), (Lepidoptera: Gelechiidae) and used of some plant extracts as insect repellents against potato tuber moth under lab. conditions. 2008

Potato tuber moth Phthorimaea operculella Zeller Lep, Gelechiidae and tuber infestation in Tunisian potato fields analysis of the flight phenology. Journal of Applied Entomology 119.5: 315-319, 1995