+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

H-2 antigen variants in a cultured heterozygous mouse leukemia cell line. VII. Effect of selection with a hybridoma antibody



H-2 antigen variants in a cultured heterozygous mouse leukemia cell line. VII. Effect of selection with a hybridoma antibody



Immunogenetics 10(5): 423-431



An (H-2k/H-2d)F1 sarcoma cell line was subjected to immunoselection using ascites fluid from a mouse growing a hybridoma secreting an anti H-2Kk antibody.One hundred random clones were picked from the surviving population and screened by direct cytolysis using the hybridoma antibody or alloantisera against H-2Kk and H-2Dk. Fifty-nine clones were resistant to all three antisera, indicating that they no longer expressed the entire H-2k haplotype. Thirty-two were resistant to the ascites and to the anti H-2Kk alloantiserum, but sensitive to the anti H-2Dk serum, indicating that they had lost H-2Kk antigen, but retained H-2Dk. Nine clones were sensitive to the alloantisera, but resistant to the hybridoma, indicating that, though they retained the product(s) recognized by the alloantiserum against H-2Kk, they had lost the site(s) that bound the hybridoma antibody. Quantitative absorption assays using lymph-node cells from young BALB.K (H-2Kk) mice as targets show that one representative clone from the last group absorbs the anti H-2Kk activity in the alloantiserum. This implies that the sensitivity of the variant clone to the alloantiserum is not due to contaminating anti C-type virus antibodies in the serum. The possible implications of these data are discussed.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 053458520

Download citation: RISBibTeXText

PMID: 22457916


Related references

H 2 antigen variants in a cultured hetero zygous mouse leukemia cell line 7. effect of selection with a hybridoma antibody. Immunogenetics 10(5): 423-432, 1980

H-2 antigen variants in a cultured heterozygous mouse leukemia cell line. VI. Rate of emergence of the hemizygous variants and evidence that the variants preexist the selection. Immunogenetics 10(5): 411-422, 1980

H-2 antigen variants in a cultured heterozygous mouse leukemia cell line. Immunogenetics 8(1): 277-281, 1979

H-2 antigen variants in a cultured heterozygous mouse leukemia cell line. Immunogenetics 5(1): 585-596, 1977

H 2 antigen variants in a cultured mouse leukemia cell line part 2 effect of selection against a k end gene. Immunogenetics 5(4): 295-308, 1977

H 2 antigen variants in a cultured hetero zygous mouse leukemia cell line part 3 effect of selection against a d end gene. Immunogenetics 5(6): 585-596, 1977

H-2 Antigen variants in a cultured heterozygous mouse leukemia cell line : IV. Cell-Mediated cytolysis of wild type and variant cells. Immunogenetics 7(1): 457-464, 1978

H-2 antigen variants in a cultured mouse leukemia cell line. Immunogenetics 5(1): 295-308, 1977

H 2 antigen variants in a cultured hetero zygous mouse leukemia cell line. Immunogenetics 4(2): 105-115, 1977

H 2 antigen variants in a cultured hetero zygous mouse leukemia cell line part 5 further cell mediated lympholysis characterization of an h 2d d negative variant. Immunogenetics 8(3): 277-282, 1979

The effect of space flight on monoclonal antibody synthesis in a hybridoma mouse cell line. Experimental Cell Research 230(2): 411-414, 1997

Production of a single chain anti-CEA antibody from the hybridoma cell line T84.66 using a modified colony-lift selection procedure to detect antigen-positive ScFv bacterial clones. Hybridoma 17(1): 1-8, 1998

A hybridoma cell line secreting antibody to poliovirus type 3 D-antigen: detection in virus harvest of two D-antigen populations. Journal of General Virology 54(Pt 2): 437-442, 1981

A hybridoma cell line secreting antibody to poliovirus type 3 d antigen detection in virus harvest of 2 d antigen populations. Journal of General Virology 54(2): 437-442, 1981

A cell surface antigen (BAL) defined by a mouse monoclonal antibody inducing apoptosis in a human lymphocytic leukemia cell line. International Journal of Cancer 57(4): 544-552, 1994