Heparin/heparan sulfate controls fibrillin-1, -2 and -3 self-interactions in microfibril assembly

Sabatier, L.; Djokic, J.; Hubmacher, D.; Dzafik, D.; Nelea, V.; Reinhardt, D.P.

Febs Letters 588(17): 2890-2897


ISSN/ISBN: 1873-3468
PMID: 25034023
DOI: 10.1016/j.febslet.2014.06.061
Accession: 053513587

Download citation:  

Article/Abstract emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Fibrillins form multifunctional microfibrils in most connective tissues. Deficiencies in fibrillin assembly can result in fibrillinopathies, such as Marfan syndrome. We demonstrate the presence of heparin/heparan sulfate binding sites in fibrillin-2 and -3. Multimerization of all three fibrillins drastically increased the apparent affinity of their interaction with heparin/heparan sulfate. Surprisingly, contrary to other reports heparin/heparan sulfate strongly inhibited homo- and heterotypic N-to-C-terminal fibrillin interactions. These data suggest that heparin/heparan sulfate controls the formation of microfibrils at the bead interaction stage.