Heparin/heparan sulfate controls fibrillin-1, -2 and -3 self-interactions in microfibril assembly

Sabatier, L.; Djokic, J.; Hubmacher, D.; Dzafik, D.; Nelea, V.; Reinhardt, D.P.

Febs Letters 588(17): 2890-2897

2014


ISSN/ISBN: 1873-3468
PMID: 25034023
DOI: 10.1016/j.febslet.2014.06.061
Accession: 053513587

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Abstract
Fibrillins form multifunctional microfibrils in most connective tissues. Deficiencies in fibrillin assembly can result in fibrillinopathies, such as Marfan syndrome. We demonstrate the presence of heparin/heparan sulfate binding sites in fibrillin-2 and -3. Multimerization of all three fibrillins drastically increased the apparent affinity of their interaction with heparin/heparan sulfate. Surprisingly, contrary to other reports heparin/heparan sulfate strongly inhibited homo- and heterotypic N-to-C-terminal fibrillin interactions. These data suggest that heparin/heparan sulfate controls the formation of microfibrils at the bead interaction stage.