+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

High concentrations of morphine sensitize and activate mouse dorsal root ganglia via TRPV1 and TRPA1 receptors

High concentrations of morphine sensitize and activate mouse dorsal root ganglia via TRPV1 and TRPA1 receptors

Molecular Pain 5: 17

Morphine and its derivatives are key drugs in pain control. Despite its well-known analgesic properties morphine at high concentrations may be proalgesic. Particularly, short-lasting painful sensations have been reported upon dermal application of morphine. To study a possible involvement of TRP receptors in the pro-nociceptive effects of morphine (0.3 - 10 mM), two models of nociception were employed using C57BL/6 mice and genetically related TRPV1 and TRPA1 knockout animals, which were crossed and generated double knockouts. Hindpaw skin flaps were used to investigate the release of calcitonin gene-related peptide indicative of nociceptive activation. Morphine induced release of calcitonin gene-related peptide and sensitized the release evoked by heat or the TRPA1 agonist acrolein. Morphine activated HEK293t cells transfected with TRPV1 or TRPA1. Activation of C57BL/6 mouse dorsal root ganglion neurons in culture was investigated with calcium imaging. Morphine induced a dose-dependent rise in intracellular calcium in neurons from wild-type animals. In neurons from TRPV1 and TRPA1 knockout animals activation by morphine was markedly reduced, in the TRPV1/A1 double knockout animals this morphine effect was abrogated. Naloxone induced an increase in calcium levels similar to morphine. The responses to both morphine and naloxone were sensitized by bradykinin. Nociceptor activation and sensitization by morphine is conveyed by TRPV1 and TRPA1.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 053533000

Download citation: RISBibTeXText

PMID: 19371406

DOI: 10.1186/1744-8069-5-17

Related references

Long-term anti-itch effect of botulinum neurotoxin A is associated with downregulation of TRPV1 and TRPA1 in the dorsal root ganglia in mice. Neuroreport 28(9): 518-526, 2017

Long-Term Activation of Group I Metabotropic Glutamate Receptors Increases Functional TRPV1-Expressing Neurons in Mouse Dorsal Root Ganglia. Frontiers in Cellular Neuroscience 10: 79, 2016

Characterization of A-425619 at native TRPV1 receptors: a comparison between dorsal root ganglia and trigeminal ganglia. European Journal of Pharmacology 596(1-3): 62-69, 2008

Sulphur-containing compounds of durian activate the thermogenesis-inducing receptors TRPA1 and TRPV1. Food Chemistry 157: 213-220, 2014

Expressions of TRPV1 and TRPA1 in the dorsal root ganglion in the rat model of orchialgia. Zhonghua Nan Ke Xue 23(4): 296-301, 2017

Peripheral effects of morphine and expression of μ-opioid receptors in the dorsal root ganglia during neuropathic pain: nitric oxide signaling. Molecular Pain 7: 25, 2011

Morphine and Pain-Related Stimuli Enhance Cell Surface Availability of Somatic -Opioid Receptors in Rat Dorsal Root Ganglia. Journal of Neuroscience 26(3): 3-62, 2006

Morphine and pain-related stimuli enhance cell surface availability of somatic delta-opioid receptors in rat dorsal root ganglia. Journal of Neuroscience 26(3): 953-962, 2006

Upregulation of miR-375 level ameliorates morphine analgesic tolerance in mouse dorsal root ganglia by inhibiting the JAK2/STAT3 pathway. Journal of Pain Research 10: 1279-1287, 2017

Increased TRPA1, TRPM8, and TRPV2 expression in dorsal root ganglia by nerve injury. Biochemical and Biophysical Research Communications 358(4): 1058-1064, 2007

Nitro-oleic acid inhibits firing and activates TRPV1- and TRPA1-mediated inward currents in dorsal root ganglion neurons from adult male rats. Journal of Pharmacology and Experimental Therapeutics 333(3): 883-895, 2010

Downregulation of miR-219 enhances brain-derived neurotrophic factor production in mouse dorsal root ganglia to mediate morphine analgesic tolerance by upregulating CaMKIIγ. Molecular Pain 12:, 2016

Opiate receptors in cultures of fetal mouse dorsal root ganglia (DRG) and spinal cord: predominance in DRG neurites. Brain Research 145(2): 396-400, 1978

Accumulation of nerve growth factor and its receptors in the uterus and dorsal root ganglia in a mouse model of adenomyosis. Reproductive Biology and Endocrinology 9: 30, 2011

The role of retinoic acid receptors in neurite outgrowth from different populations of embryonic mouse dorsal root ganglia. Journal of Cell Science 113: 2567-2574, 2000