+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

High-throughput small molecule identification using MALDI-TOF and a nanolayered substrate



High-throughput small molecule identification using MALDI-TOF and a nanolayered substrate



Analytical Chemistry 83(13): 5283-5289



Encoderless combinatorial chemistry requires high-throughput product identification without the use of chemical or other tags. We developed a novel nanolayered substrate plate and combined it with a microarraying robot, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, and custom software to produce a high-throughput small molecule identification system. To optimize system performance, we spotted 5 different chemical entities, spanning a m/z range of 195 to 1338, in 20,304 spots for a total of 101,520 molecules. The initial spot identification rate was 99.85% (20,273 spots), and after a proofreading algorithm was added, 100% of 20,304 spots and 101,520 molecules were identified. An internal recalibration algorithm also significantly improved mass accuracy to as low as 45 ppm. Using this optimized system, 47 different chemical entities, spanning a m/z range of 138 to 1,592, were spotted over 5,076 spots and could be identified with 100% accuracy. Our study lays the foundation for improved encoderless combinatorial chemistry.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053553163

Download citation: RISBibTeXText

PMID: 21651231

DOI: 10.1021/ac2006735


Related references

Identification of promethazine as an amyloid-binding molecule using a fluorescence high-throughput assay and MALDI imaging mass spectrometry. Neuroimage. Clinical 2: 620-629, 2013

High-throughput workflow for identification of phosphorylated peptides by LC-MALDI-TOF/TOF-MS coupled to in situ enrichment on MALDI plates functionalized by ion landing. Journal of Mass Spectrometry 50(6): 802-811, 2016

Identification of Small-Molecule Frequent Hitters from AlphaScreen High-Throughput Screens. Journal of Biomolecular Screening 19(5): 715-726, 2013

A high throughput screening assay system for the identification of small molecule inhibitors of gsp. Plos One 9(3): E90766-E90766, 2015

High-throughput screening for the identification of small-molecule inhibitors of the flaviviral protease. Antiviral Research 134(): 6-16, 2016

Identification of small molecule inhibitors of p27Kip1 ubiquitination by high-throughput screening. 2013

Identification of small molecule inhibitors of p27(Kip1) ubiquitination by high-throughput screening. Cancer Science 104(11): 1461-1467, 2014

Identification of promiscuous small molecule activators in high-throughput enzyme activation screens. Journal of Medicinal Chemistry 51(8): 2346-2349, 2008

Identification of small molecule NPR-B antagonists by high throughput screening--potential use in heart failure. Naunyn-Schmiedeberg's Archives of Pharmacology 387(1): 5-14, 2014

Identification of small-molecule inhibitors of protein kinase B (PKB/AKT) in an AlphaScreenTM high-throughput screen. Journal of Biomolecular Screening 11(7): 822-827, 2006

Microarray-assisted high-throughput identification of a cell-permeable small-molecule binder of 14-3-3 proteins. Angewandte Chemie 49(37): 6528-6532, 2011

Identification of novel quadruplex ligands from small molecule libraries by FRET-based high-throughput screening. Chembiochem 12(9): 1422-1426, 2011

High-throughput screening for the identification of small-molecule inhibitors of retinoblastoma protein phosphorylation in cells. Analytical Biochemistry 320(1): 66-74, September 1, 2003

Identification of novel small-molecule inhibitors of glioblastoma cell growth and invasion by high-throughput screening. Bioscience Trends 6(4): 192-200, 2013