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Identification of a cell-bound extracellular protease overproduced by Sulfolobus solfataricus in peptide-rich media



Identification of a cell-bound extracellular protease overproduced by Sulfolobus solfataricus in peptide-rich media



Protein and Peptide Letters 17(1): 78-85



A new protease, named SsMTP was identified from the archeon Sulfolobus solfataricus. The enzyme is associated to the cell-membrane and over-produced in response to the peptide-enriched media. SsMTP has a molecular mass of 120 kDa showing optimal activity at pH 2.0 in the temperature range 70 - 90 degrees C, and a half-life of 20 days at 80 degrees C. Primary structure analysis revealed that SsMTP represents a novel type of multi-domain thermopsin-like protease containing the catalytic domain followed by two distinct domains, PKD and Y_Y_Y, which are usually involved in a range of protein-protein interactions among the extracellular proteins.

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Accession: 053660499

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PMID: 19508193


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