+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Identification of a novel skin penetration enhancement peptide by phage display peptide library screening



Identification of a novel skin penetration enhancement peptide by phage display peptide library screening



Molecular Pharmaceutics 9(5): 1320-1330



Skin is an important site for local or systemic application of drugs. However, a majority of drugs have poor permeability through the skin's topmost layer, stratum corneum (SC). The aim of this study was to identify safe and smaller peptides that could enhance the skin penetration of drug molecules. By screening phage display peptide library, we have identified a T2 peptide (LVGVFH), which enhanced the penetration of bacteriophages (~800 nm long bacterial viruses) across porcine and mouse skin. Pretreating the skin with synthetic T2 peptide at pH 4.5 resulted in significant penetration enhancement of hydrophilic drug 5-fluorouracil (5-FU) across skin. FTIR spectroscopy showed that the T2 peptide interacted with skin lipids to enhance the skin penetration. Pretreating the skin with T2 peptide enhanced the partitioning of small molecules with different lipophilicities (5-FU, fluorescein isothiocyanate, and rhodamine 123 hydrochloride) into skin. Fluorescence studies showed that T2 peptide enhanced the diffusion of these molecules into intercellular lipids of SC and thus enhanced the penetration into the skin. Histidine at the c-terminus of T2 peptide was identified to be critical for the skin penetration enhancement. T2 peptide interacted with skin lipids to cause skin penetration enhancement. The study identified a novel, safe, and noninvasive peptide to improve the skin penetration of drugs without chemical conjugation.

(PDF emailed within 0-6 h: $19.90)

Accession: 053662021

Download citation: RISBibTeXText

PMID: 22452335

DOI: 10.1021/mp200594z


Related references

Screening and identification of a targeting peptide to hepatocarcinoma from a phage display peptide library. Molecular Medicine 13(5-6): 246-254, 2007

Screening and identification of a renal carcinoma specific peptide from a phage display peptide library. Journal of Experimental & Clinical Cancer Research 30: 105-105, 2012

Screening and identification of a peptide specifically targeted to NCI-H1299 from a phage display peptide library. Cancer Letters 281(1): 64-70, 2009

Retraction: Screening and identification of a renal carcinoma specific peptide from a phage display peptide library. Journal of Experimental & Clinical Cancer Research 31: 21-21, 2012

Screening and identification of a peptide specifically targeted to NCI-H1299 cells from a phage display peptide library. Molecular Medicine Reports 2(6): 1005-1010, 2009

Identification of a peptide specifically targeting ovarian cancer by the screening of a phage display peptide library. Oncology Letters 11(6): 4022-4026, 2016

Screening and identification of a specific peptide binding to hepatocellular carcinoma cells from a phage display peptide library. Journal of Peptide Science 20(3): 196-202, 2014

Screening and identification of polypeptides specifically binding to human B type natriuretic peptide from 12TM phage display peptide library. Nan Fang Yi Ke Da Xue Xue Bao 29(9): 1837-1839, 2011

Screening and identification of CD13-binding peptides with phage display peptide library. Nan Fang Yi Ke Da Xue Xue Bao 30(4): 827-830, 2011

Identification of calreticulin as a ligand of GABARAP by phage display screening of a peptide library. Febs Journal 274(21): 5543-5555, 2007

Screening of peptide inhibitors of acetylcholinesterase from 12-mer random phage display peptide library. Nan Fang Yi Ke Da Xue Xue Bao 26(7): 1053-1054, 2006

Screening of TACE peptide inhibitors from phage display peptide library. Journal of Huazhong University of Science and Technology. Medical Sciences 25(5): 473-476, 2006

Screening of antagonist peptide of BLyS from C7C phage display peptide library. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 20(4): 398-401, 2004

Screening of a specific peptide binding to VPAC1 receptor from a phage display peptide library. Plos One 8(1): E54264-E54264, 2013

Screening and identification of a novel hepatocellular carcinoma cell binding peptide by using a phage display library. Journal of Huazhong University of Science and Technology. Medical Sciences 28(3): 299-303, 2008