EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Identification of centromeric and telomeric DNA-binding proteins in rice


Identification of centromeric and telomeric DNA-binding proteins in rice



Proteomics 13(5): 826-832



ISSN/ISBN: 1615-9853

PMID: 23303719

DOI: 10.1002/pmic.201100416

Centromeres and telomeres are DNA/protein complexes and essential functional components of eukaryotic chromosomes. Previous studies have shown that rice centromeres and telomeres are occupied by CentO (rice centromere satellite DNA) satellite and G-rich telomere repeats, respectively. However, the protein components are not fully understood. DNA-binding proteins associated with centromeric or telomeric DNAs will most likely be important for the understanding of centromere and telomere structure and functions. To capture DNA-specific binding proteins, affinity pull-down technique was applied in this study to isolate rice centromeric and telomeric DNA-binding proteins. Fifty-five proteins were identified for their binding affinity to rice CentO repeat, and 80 proteins were identified for their binding to telomere repeat. One CentO-binding protein, Os02g0288200, was demonstrated to bind to CentO specifically by in vitro assay. A conserved domain, DUF573 with unknown functions was identified in this protein, and proven to be responsible for the specific binding to CentO in vitro. Four proteins identified as telomere DNA-binding proteins in this study were reported by different groups previously. These results demonstrate that DNA affinity pull-down technique is effective in the isolation of sequence-specific binding proteins and will be applicable in future studies of centromere and telomere proteins.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053664124

Download citation: RISBibTeXText

Related references

Cytosine methylation changes in rice centromeric and telomeric sequences induced by foreign DNA introgression. Biologia Plantarum 44(2): 203-206, 2001

TeCK database: a comprehensive collection of telomeric and centromeric sequences with their associated proteins. Bioinformation 2(2): 73-75, 2007

Identification of non-telomeric G4-DNA binding proteins in human, E. coli, yeast, and Arabidopsis. Molecules and Cells 14(3): 404-410, 2002

Identification and characterization of a large subset of human PAC clones with centromeric and telomeric probes. Cytogenetics and Cell Genetics 77(1-2): 27, 1997

Identification, characterisation and clinical applications of cosmids from the telomeric and centromeric regions of the long arm of chromosome 22. Human Genetics 94(4): 339-345, 1994

Searching for a common centromeric structural motif: Drosophila centromeric satellite DNAs show propensity to form telomeric-like unusual DNA structures. Genetica 109(1-2): 71-75, 2000

Actin-binding protein (ABP-280) filamin gene (FLN) maps telomeric to the color vision locus (R/GCP) and centromeric to G6PD in Xq28. Genomics 17(2): 496-498, 1993

Purification and characterization of ten new rice NaCl-soluble proteins: identification of four protein-synthesis inhibitors and two immunoglobulin-binding proteins. Planta 181(1): 1-9, 1990

Purification and characterization of ten new rice sodium chloride soluble proteins identification of four protein synthesis inhibitors and two immunoglobulin binding proteins. Planta (Heidelberg) 181(1): 1-9, 1990

Identification and expression analysis of odorant binding proteins and chemosensory proteins from dissected antennae and mouthparts of the rice bug Leptocorisa acuta. Comparative Biochemistry and Physiology. Part D Genomics and Proteomics 33: 100631, 2020

The telomeric poly(ADP-ribose) polymerase, tankyrase 1, contains multiple binding sites for telomeric repeat binding factor 1 (TRF1) and a novel acceptor, 182-kDa tankyrase-binding protein (TAB182). Journal of Biological Chemistry 277(16): 14116-14126, 2002

Arabidopsis thaliana telomeric DNA-binding protein 1 is required for telomere length homeostasis and its Myb-extension domain stabilizes plant telomeric DNA binding. Nucleic Acids Research 35(4): 1333-1342, 2007

Identification of cadmium-binding proteins from rice (Oryza sativa L.). International Journal of Biological Macromolecules 119: 597-603, 2018

Telomeric DNA binding proteins. Bioessays: News and Reviews in Molecular Cellular and Developmental Biology 15(8): 555-557, 1993

Identification of Cu-binding proteins in embryos of germinating rice in response to Cu toxicity. Acta Physiologiae Plantarum 40(8): 158, 2018