+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification of phosphorylation sites in phosphopeptides by positive and negative mode electrospray ionization-tandem mass spectrometry

Identification of phosphorylation sites in phosphopeptides by positive and negative mode electrospray ionization-tandem mass spectrometry

Journal of the American Society for Mass Spectrometry 7(3): 243-249

A series of synthetic mono- and diphosphorylated peptides has been analyzed by positive and negative mode electrospray ionization-tandem mass spectrometry. The synthetic peptides are serine- and threonine-phosphorylated analogs of proteolytic fragments from the C-terminal region of rhodopsin. Use of positive and negative modes of electrospray ionization to produce ions for tandem mass spectrometry via low energy collision-induced dissociation was explored. For some of the peptides, the complementary use of experimental results allowed determination of the phosphorylation sites when either mode alone gave incomplete information. Other peptides, however, gave negative ion spectra not interpretable in terms of backbone cleavages. However, use of positive ion tandem mass spectrometry of different charge state precursor ions gave sufficient information in most cases to assign sites of phosphorylation. These results illustrate the utility of obtaining complementary information by tandem mass spectrometry by using precursor ions of different charge polarity or number.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 053669656

Download citation: RISBibTeXText

PMID: 24203295

DOI: 10.1016/1044-0305(95)00675-3

Related references

Identification of phosphorylation sites in insulin receptor substrate-1 by hypothesis-driven high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. Analytical Chemistry 77(17): 5693-5699, 2005

Simultaneous identification of nine carcinogenic dyes from textiles by liquid chromatography/electrospray ionization mass spectrometry via negative/positive ion switching mode. European Journal of Mass Spectrometry 15(6): 705-713, 2009

Atmospheric solid analysis probe mass spectrometry vs electrospray tandem mass spectrometry of polydimethylsiloxanes in positive and negative ionization modes. Rapid Communications in Mass Spectrometry 29(10): 982-986, 2015

Analysis of curcuminoids by positive and negative electrospray ionization and tandem mass spectrometry. Rapid Communications in Mass Spectrometry 20(6): 1001-1012, 2006

Identification of phosphorylated proteins from thrombin-activated human platelets isolated by two-dimensional gel electrophoresis by electrospray ionization-tandem mass spectrometry (ESI-MS/MS) and liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). Electrophoresis 19(6): 1015-1023, 1998

Polyalanine - a practical polypeptide mass calibration standard for matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry in positive and negative mode. Rapid Communications in Mass Spectrometry 30(6): 681-683, 2016

Structural characterization and identification of dibenzocyclooctadiene lignans in Fructus Schisandrae using electrospray ionization ion trap multiple-stage tandem mass spectrometry and electrospray ionization Fourier transform ion cyclotron resonance multiple-stage tandem mass spectrometry. Analytica Chimica Acta 615(2): 124-135, 2008

Tandem mass spectrometry of poly(methacrylic Acid) oligomers produced by negative mode electrospray ionization. Journal of the American Society for Mass Spectrometry 20(1): 25-33, 2008

Titania as a chemo-affinity support for the column-switching HPLC analysis of phosphopeptides: application to the characterization of phosphorylation sites in proteins by combination with protease digestion and electrospray ionization mass spectrometry. Analytical Sciences 20(5): 861-864, 2004

Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry as an alternative proteomics platform to ultraperformance liquid chromatography-electrospray ionization-tandem mass spectrometry for samples of intermediate complexity. Analytical Chemistry 84(3): 1617-1622, 2012

Analysis of phytochelatin-cadmium complexes from plant tissue culture using nano-electrospray ionization tandem mass spectrometry and capillary liquid chromatography/electrospray ionization tandem mass spectrometry. Journal of Mass Spectrometry 34(9): 930-941, 1999

Simultaneous determination of banned acid orange dyes and basic orange dyes in foodstuffs by liquid chromatography-tandem electrospray ionization mass spectrometry via negative/positive ion switching mode. Journal of Agricultural and Food Chemistry 61(16): 3834-3841, 2014

Identification of two-dimensionally separated human cerebrospinal fluid proteins by N-terminal sequencing, matrix-assisted laser desorption/ionization - mass spectrometry, nanoliquid chromatography-electrospray ionization-time of flight-mass spectrometry, and tandem mass spectrometry. Electrophoresis 21(11): 2266-2283, 2000

Determination and quantification of camptothecin in an endophytic fungus by liquid chromatography - positive mode electrospray ionization tandem mass spectrometry. Current Science 91(2): 208-212, 2006

Negative and positive ion matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and positive ion nano-electrospray ionization quadrupole ion trap mass spectrometry of peptidoglycan fragments isolated from various Bacillus species. Journal of Mass Spectrometry 36(2): 124-139, 2001