+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Identification of the transcription factor Znc1p, which regulates the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica



Identification of the transcription factor Znc1p, which regulates the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica



Plos One 8(6): E66790



The dimorphic yeast Yarrowia lipolytica is used as a model to study fungal differentiation because it grows as yeast-like cells or forms hyphal cells in response to changes in environmental conditions. Here, we report the isolation and characterization of a gene, ZNC1, involved in the dimorphic transition in Y. lipolytica. The ZNC1 gene encodes a 782 amino acid protein that contains a Zn(II)2C6 fungal-type zinc finger DNA-binding domain and a leucine zipper domain. ZNC1 transcription is elevated during yeast growth and decreases during the formation of mycelium. Cells in which ZNC1 has been deleted show increased hyphal cell formation. Znc1p-GFP localizes to the nucleus, but mutations within the leucine zipper domain of Znc1p, and to a lesser extent within the Zn(II)2C6 domain, result in a mislocalization of Znc1p to the cytoplasm. Microarrays comparing gene expression between znc1::URA3 and wild-type cells during both exponential growth and the induction of the yeast-to-hypha transition revealed 1,214 genes whose expression was changed by 2-fold or more under at least one of the conditions analyzed. Our results suggest that Znc1p acts as a transcription factor repressing hyphal cell formation and functions as part of a complex network regulating mycelial growth in Y. lipolytica.

(PDF emailed within 0-6 h: $19.90)

Accession: 053672769

Download citation: RISBibTeXText

PMID: 23826133

DOI: 10.1371/journal.pone.0066790


Related references

The TEA/ATTS transcription factor YlTec1p represses yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica. FEMS Yeast Research, 2012

The TEA/ATTS transcription factor YlTec1p represses the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica. Fems Yeast Research 13(1): 50-61, 2015

Serum-induced hypha formation in the dimorphic yeast Yarrowia lipolytica. FEMS microbiology letters, 2000 190(1): 9-12, 2000

Induction of the yeast-hypha transition in Yarrowia lipolytica. Wolf, Klaus , Reprint Author, Breunig, Karin, Barth, Gerold Non-conventional yeasts in genetics, biochemistry and biotechnology: 339-342, 2003

Proteomic analysis reveals metabolic changes during yeast to hypha transition in Yarrowia lipolytica. Journal of Mass Spectrometry 42(11): 1453-1462, 2007

Roles of the three Ras proteins in the regulation of dimorphic transition in the yeast Yarrowia lipolytica. Fems Yeast Research 14(3): 451-463, 2014

A phosphatidylinositol/phosphatidylcholine transfer protein is required for differentiation of the dimorphic yeast Yarrowia lipolytica from the yeast to the mycelial form. Journal Of Cell Biology. 125(1): 113-127, 1994

MHY1 encodes a C2H2-type zinc finger protein that promotes dimorphic transition in the yeast Yarrowia lipolytica. Journal of Bacteriology 181(10): 3051-3057, 1999

Peroxisomal peripheral membrane protein YlInp1p is required for peroxisome inheritance and influences the dimorphic transition in the yeast Yarrowia lipolytica. Eukaryotic Cell 6(9): 1528-1537, 2007

Basic helix-loop-helix transcription factor heterocomplex of Yas1p and Yas2p regulates cytochrome P450 expression in response to alkanes in the yeast Yarrowia lipolytica. Eukaryotic Cell 6(4): 734-743, 2007

Development of the dimorphic yeast yarrowia lipolytica. Journal of Cellular Biochemistry Supplement (9 PART C): 193, 1985

One-step transformation of the dimorphic yeast Yarrowia lipolytica. Applied microbiology and biotechnology 48(2): 232-235, 1997

Development of the genetics of the dimorphic yeast Yarrowia lipolytica. UCLA symposia on molecular and cellular biology4(34): 367-381, 1985

Ylli, a non-LTR retrotransposon L1 family in the dimorphic yeast Yarrowia lipolytica. Molecular Biology & Evolution 19(5): 664-677, 2002

Cloning and characterisation of the ribosomal RNA genes of the dimorphic yeast, Yarrowia lipolytica. Current Genetics 10(6): 449-452, 1986