EurekaMag.com logo
+ Site Statistics
References:
53,517,315
Abstracts:
29,339,501
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Single mRNA Particle Tracking with ImageJ-Based Analysis



Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Single mRNA Particle Tracking with ImageJ-Based Analysis



Csh Protocols 2007: Pdb.Prot4872-Pdb.Prot4872



INTRODUCTIONThis protocol describes the use of ImageJ software (freely available from NIH) to analyze particle dynamics in a cell using time-lapse movie frames or image stacks of fluorescent mRNA particles. Maximum intensity projections and kymographs are produced.

(PDF 0-2 workdays service: $29.90)

Accession: 053686883

Download citation: RISBibTeXText

PMID: 21356980



Related references

Imaging real-time gene expression in living systems with single-transcript resolution: image analysis of single mRNA transcripts. Csh Protocols 2007: Pdb.Prot4871-Pdb.Prot4871, 2007

Imaging real-time gene expression in living systems with single-transcript resolution: construct design and imaging system setup. Csh Protocols 2007: Pdb.Top28-Pdb.Top28, 2007

Imaging real-time gene expression in Mammalian cells with single-transcript resolution. Csh Protocols 2007: Pdb.Prot4869-Pdb.Prot4869, 2007

Real-time imaging of gene expression in single living cells. Chemistry & Biology (London) 5(11): R285, 1998

Single particle tracking-based reaction progress kinetic analysis reveals a series of molecular mechanisms of cetuximab-induced EGFR processes in a single living cell. Chemical Science 8(7): 4823-4832, 2017

Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking. Chemical Reviews 117(11): 7244-7275, 2017

Quantitative real-time analysis of HIV-1 gene expression dynamics in single living primary cells. Biotechnology Journal 1(6): 682-689, 2006

Super-resolution imaging-based single particle tracking reveals dynamics of nanoparticle internalization by live cells. Nanoscale 8(14): 7423-7429, 2016

His-tag based in situ labelling of progeny viruses for real-time single virus tracking in living cells. Nanoscale 8(44): 18635-18639, 2016

Functioning nanomachines seen in real-time in living bacteria using single-molecule and super-resolution fluorescence imaging. International Journal of Molecular Sciences 12(4): 2518-2542, 2011

Single-pair fluorescence resonance energy transfer analysis of mRNA transcripts for highly sensitive gene expression profiling in near real time. Analytical Chemistry 85(16): 7851-7858, 2014

A Protocol for Real-time 3D Single Particle Tracking. Journal of Visualized Experiments 131), 2018

Real-time nanomicroscopy via three-dimensional single-particle tracking. Chemphyschem 10(14): 2458-2464, 2009

Far-field photostable optical nanoscopy (PHOTON) for real-time super-resolution single-molecular imaging of signaling pathways of single live cells. Nanoscale 4(9): 2797-2812, 2012

Quantum dot-DNA origami binding: a single particle, 3D, real-time tracking study. Chemical Communications 49(9): 907-909, 2013